Molecular identification of miR-145 and miR-9 expression level as prognostic biomarkers for early-stage cervical cancer detection

Azizmohammadi, S. and Safari, A. and Azizmohammadi, S. and Kaghazian, M. and Sadrkhanlo, M. and Yahaghi, E. and Farshgar, R. and Seifoleslami, M. (2017) Molecular identification of miR-145 and miR-9 expression level as prognostic biomarkers for early-stage cervical cancer detection. QJM, 110 (1). pp. 11-15.

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Abstract

Background: MicroRNAs (miRNAs) may act as carcinogen or tumor suppressor genes by targeting various biological molecules. Therefore, it is important to identify significant markers for prognosis, diagnosis treatment strategies of cancers. Objective: To evaluate the clinical importance and prognostic value of miR-9 and miR-145 in cervical cancer. Method: miRNAs expression was detected using quantitative real-time polymerase chain reaction in cervical cancer specimens and adjacent normal tissues. Results: MiR-9 up-regulated in cervical cancer specimens than adjacent normal tissues (9.74362.172 vs. 2.13161.083; P < 0.05). MiR-145 was decreased in cervical cancer specimens compared to corresponding normal tissues (2.18960. 724 vs. 7.17361.558 P < 0.05). In addition, increased expression of miR-9 was strongly linked to lymph node metastasis (P =0.017) and vascular invasion (P =0.011). On the other hand, the low expression of miR-145 was related to advanced FIGO stage (P =0.007), lymph node metastasis (P =0.02) and vascular invasion (0.026). Kaplan-Meier survival and log-rank analysis suggested that patients with high expression of miR-9 had shorter overall survival compared with those with low expression (log-rank test P =0.028; P < 0.001). In addition, shorter overall survival time was remarkably linked to decreased expression of miR-145 (log-rank test P < 0.001). Multivariate Cox proportional hazards model analysis of miR-9 and miR-145 showed that FIGO stage (P =0.011) high expression of miR-9 and low expression of miR-145 (P=0.023; P =0.031) were independent prognostic factors for overall survival of patients. Conclusions: miRNA-145 and 9 may be as potential prognostic marker in patients suffering from cervical cancer. © The Author 2016.

Item Type: Article
Additional Information: Cited By :21 Export Date: 16 February 2020 CODEN: QMJPF Correspondence Address: Seifoleslami, M.; Department of Gynecology, Khanevadeh Hospital, AJA University of Medical SciencesIran; email: mehriseifoleslami@yahoo.com.sg
Uncontrolled Keywords: biological marker microRNA 145 microRNA 9 tumor marker microRNA MIRN145 microRNA, human MIRN92 microRNA, human adult advanced cancer aged Article cancer prognosis cancer staging cancer survival clinical article controlled study early cancer early cancer diagnosis evaluation study female gene expression human human tissue lymph node metastasis lymph vessel metastasis middle aged molecular diagnosis overall survival priority journal quantitative analysis real time polymerase chain reaction upregulation uterine cervix cancer genetics Kaplan Meier method multivariate analysis procedures prognosis proportional hazards model Uterine Cervical Neoplasms very elderly Aged, 80 and over Biomarkers, Tumor Early Detection of Cancer Humans Kaplan-Meier Estimate Lymphatic Metastasis MicroRNAs Proportional Hazards Models Up-Regulation
Subjects: WP Gynecology
QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 04:01
Last Modified: 23 May 2020 05:00
URI: http://eprints.mums.ac.ir/id/eprint/16761

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