Coencapsulation of CpG oligodeoxynucleotides with recombinant Leishmania major stress-inducible protein 1 in liposome enhances immune response and protection against leishmaniasis in immunized BALB/c mice

Badiee, A. and Jaafari, M. R. and Samiei, A. and Soroush, D. and Khamesipour, A. (2008) Coencapsulation of CpG oligodeoxynucleotides with recombinant Leishmania major stress-inducible protein 1 in liposome enhances immune response and protection against leishmaniasis in immunized BALB/c mice. Clinical and Vaccine Immunology, 15 (4). pp. 668-674.

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Abstract

CpG oligodeoxynucleotides (CpG ODN) have been shown to have potent adjuvant activity for a wide range of antigens. The purpose of this study was to determine the potential benefit of using liposomes as a delivery vehicle to enhance the adjuvant activity of CpG ODN with Leishmania major stress-inducible protein 1 (LmSTI1) antigen in induction of the Th1 response in a murine model of leishmaniasis. BALB/c mice were immunized subcutaneously three times in 3-week intervals with liposomal recombinant LmSTI1 (Lip-rLmSTI1), rLmSTI1 coencapsulated with CpG ODN in a liposome (Lip-rLmSTI1-CpG ODN), rLmSTI1 plus CpG ODN in phosphate-buffered saline (PBS), rLmSTI1 plus non-CpG ODN in PBS, rLmSTI1 in PBS, empty liposome, or PBS. The intensity of infection induced by L. major promastigote challenge was measured by footpad swelling. A significant (P < 0.001) inhibition of infection in mice immunized with Lip-rLmSTI1-CpG ODN was shown compared to the other groups, and no parasite was detected in the spleens of this group 14 weeks after challenge. The highest immunoglobulin G2a (IgG2a) titer and the highest IgG2a/IgG1 ratio were also shown in the sera of mice immunized with Lip-rLmSTI1-CpG ODN before and 14 weeks after challenge. The results indicated the superiority of CpG ODN in its liposomal form over its soluble form to induce the Th1 response when used in association with rLmSTI1 antigen. It seems that using a liposome delivery system carrying CpG ODN as an adjuvant coencapsulated with Leishmania antigen plays an important role in vaccine development strategies against leishmaniasis. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Item Type: Article
Additional Information: Cited By :35 Export Date: 16 February 2020 Correspondence Address: Khamesipour, A.; Center for Research and Training in Skin Diseases and Leprosy, Medical Sciences/University of Tehran, P.O. Box 14155-6383, Tehran 14166, Iran; email: khamesipourali@yahoo.com
Uncontrolled Keywords: CpG oligodeoxynucleotide immunoglobulin G immunoglobulin G1 immunoglobulin G1 antibody immunoglobulin G2a immunoglobulin G2a antibody immunological adjuvant Leishmania vaccine liposome phosphate buffered saline recombinant protein recombinant stress inducible protein 1 animal cell animal experiment animal model antibody response antibody titer article controlled study drug delivery system drug potency encapsulation female foot pad immune response immunization Leishmania major leishmaniasis mouse nonhuman priority journal promastigote swelling Th1 cell Animals Antibodies, Protozoan Electrophoresis, Polyacrylamide Gel Heat-Shock Proteins Leishmaniasis, Cutaneous Liposomes Mice Mice, Inbred BALB C Oligodeoxyribonucleotides Protozoan Proteins Protozoan Vaccines Recombinant Proteins Spleen Th1 Cells
Subjects: WR Dermatology
QU Biochemistry
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 05:33
Last Modified: 18 May 2020 05:33
URI: http://eprints.mums.ac.ir/id/eprint/16762

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