Antifungal susceptibility testing of Candida species isolated from the immunocompromised patients admitted to ten university hospitals in Iran: Comparison of colonizing and infecting isolates

Badiee, P. and Badali, H. and Boekhout, T. and Diba, K. and Moghadam, A. G. and Hossaini Nasab, A. and Jafarian, H. and Mohammadi, R. and Mirhendi, H. and Najafzadeh, M. J. and Shamsizadeh, A. and Soltani, J. (2017) Antifungal susceptibility testing of Candida species isolated from the immunocompromised patients admitted to ten university hospitals in Iran: Comparison of colonizing and infecting isolates. BMC Infectious Diseases, 17 (1).

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Abstract

Background: Antifungal susceptibility testing is a subject of interest in the field of medical mycology. The aim of the present study were the distributions and antifungal susceptibility patterns of various Candida species isolated from colonized and infected immunocompromised patients admitted to ten university hospitals in Iran. Methods: In totally, 846 Candida species were isolated from more than 4000 clinical samples and identified by the API 20 C AUX system. Antifungal susceptibility testing was performed by broth microdilution method according to CLSI. Results: The most frequent Candida species isolated from all patients was Candida albicans (510/846). The epidemiological cutoff value and percentage of wild-type species for amphotericin B and fluconazole in Candida albicans, Candida tropicalis, Candida glabrata and Candida krusei were 0.5μg/ml (95) and 4μg/ml (96); 1μg/ml (95) and 8μg/ml (95); 0.5μg/ml (99) and 19μg/ml (98); and 4μg/ml (95) and 64μg/ml (95), respectively. The MIC90 and epidemiological cutoff values to posaconazole in Candida krusei were 0.5μg/ml. There were significant differences between infecting and colonizing isolates of Candida tropicalis in MIC 90 values of amphotericin B, and isolates of Candida glabrata in values of amphotericin B, caspofungin, and voriconazole (P<0.05). Conclusions: Our findings suggest that the susceptibility patterns of Candida species (colonizing and infecting isolates) in immunocompromised patients are not the same and acquired resistance was seen in some species. © 2017 The Author(s).

Item Type: Article
Additional Information: Cited By :11 Export Date: 16 February 2020 CODEN: BIDMB Correspondence Address: Badiee, P.; Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical SciencesIran; email: badieep@sums.ac.ir
Uncontrolled Keywords: Candida Albicans Candida infected patients Candida susceptibility testing Candida wild-type Colonizing Candida amphotericin B caspofungin fluconazole posaconazole voriconazole antifungal agent echinocandin lipopeptide triazole derivative abscess antifungal susceptibility Article blood broth dilution Candida Candida dubliniensis Candida famata Candida glabrata Candida intermedia Candida parapsilosis Candida tropicalis Clavispora lusitaniae controlled study cross-sectional study fungal colonization fungal strain fungus isolation human immunocompromised patient Iran Kluyveromyces marxianus lung lavage Meyerozyma guilliermondii minimum inhibitory concentration mouth nose Pichia kudriavzevii rectum sputum university hospital urine vagina antifungal resistance clinical trial comparative study drug effects female isolation and purification microbial sensitivity test multicenter study pathogenicity Antifungal Agents Cross-Sectional Studies Drug Resistance, Fungal Echinocandins Hospitals, University Humans Immunocompromised Host Lipopeptides Microbial Sensitivity Tests Triazoles
Subjects: WC Communicable Diseases
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 05:42
Last Modified: 18 May 2020 05:42
URI: http://eprints.mums.ac.ir/id/eprint/16763

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