Multicenter identification and antifungal susceptibility patterns of candida species isolated from clinical samples

Badiee, P. and Badali, H. and Diba, K. and Jafarian, H. and Mohammadi, R. and Mirhendi, H. and Najafzadeh, M. J. (2017) Multicenter identification and antifungal susceptibility patterns of candida species isolated from clinical samples. Jundishapur Journal of Microbiology, 10 (12).

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Background: Invasive fungal infections without proper treatment could lead to high mortality rate, especially in immunocompromised patients. Candida species distribution and drug susceptibility patterns vary in different areas. Understanding the etiologic agents and drug susceptibility patterns in each region are required for the best management of patients with Candida infections. Objectives: The aim of this study was to identify Candida species isolated from clinical samples of six university hospitals in Iran and detect their susceptibility patterns to seven antifungal agents. Methods: Clinical samples from patients with fungal infections were cultured on Sabouraud dextrose agar. Isolated yeasts were identified by API 20C AUX kit, according to the manufacturer’s instructions. Drug susceptibility patterns to amphotericin B, caspofungin, voriconazole, fluconazole, posaconazole, itraconazole and ketoconazole were determined, according to CLSI M27-A3 and S4. Results: In total, 428 species of Candida were isolated from clinical samples (1950 samples). Most isolated species were Candida albicans, followed by C. tropicalis, C. parapsilosis, C. kefyr, C. famata, C. glabrata, C. krusei, C. dubliniensis, C. guilliermondii and C. lusitaniae. Sensitivity rate of C. albicans to amphotericin B, caspofungin, voriconazole, fluconazole, and itraconazole was 96.6, 99.5, 88.6, 90.6, and 52 with MIC90 values equal to 0.25 µg/mL, 0.125µg/mL, 0.125 µg/mL, 2 µg/mL, and 1 µg/mL, respectively. The MIC 90 values for ketoconazole and posaconazole were 0.125µg/mL and 0.064µg/mL, respectively. Different sensitivity to antifungal agents was present in non-albicans Candida species especially in C. krusei, C. glabrata, and C. tropicalis. Conclusions: According to this study, C. albicans is the most prevalent etiologic agent in infected patients and caspofungin is the most effective antifungal agent. Knowledge about etiologic agents and their susceptibility patterns in each region is helpful for successful treatment of the patients. © 2017, Jundishapur Journal of Microbiology.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Jafarian, H.; Prof. Alborzi Clinical Microbiology Research Center, Nemazi Hospital, Zand Ave, Iran; email:
Uncontrolled Keywords: Amphotericin B Candida albicans Candida tropicalis Fluconazole Itraconazole caspofungin ketoconazole posaconazole voriconazole antifungal susceptibility Article Candida Candida dubliniensis Candida famata Candida glabrata Candida parapsilosis Clavispora lusitaniae clinical practice controlled study drug selectivity drug sensitivity fungus culture fungus identification fungus isolation human Iran Kluyveromyces marxianus Meyerozyma guilliermondii MIC90 multicenter study mycosis nonhuman Pichia kudriavzevii sample university hospital yeast
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 03:58
Last Modified: 18 May 2020 03:58

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