Comparative analysis of selected cagPAI genes and different vaca genotypes in Iranian and Turkish H. Pylori-positive patients suffering from gastric adenocarcinoma and active chronic gastritis

Bahadori, A. and Somi, M. H. and Doran, F. and Eftekharsadat, A. and Kizilyildirim, S. and Esmaeillou, M. and Yaasi, M. and Bedır, B. and Köksal, F. (2017) Comparative analysis of selected cagPAI genes and different vaca genotypes in Iranian and Turkish H. Pylori-positive patients suffering from gastric adenocarcinoma and active chronic gastritis. Turkish Journal of Medical Sciences, 47 (3). pp. 916-922.

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Abstract

Background/aim: Helicobacter pylori is a pathogen that colonizes a majority of the world’s population. Genetic diversity within the virulence genes of bacteria such as cagPAI and vacA may have a modified effect on the pathogenic potential of the bacteria. This study aimed to investigate which genes can be suggested as potentially related virulence factors for H. pylori-associated active chronic gastritis and stomach adenocarcinoma in the northwest of Iran and south of Turkey. Materials and methods: Formalin-fixed, paraffin-embedded stomach biopsy tissue samples were obtained from Iranian and Turkish patients from selected geographical regions. The prevalence of selected cagPAI genes and vacA genotypes were studied in H. pylori-positive samples by using polymerase chain reaction and specific primers. Results: Out of 320 patients, H. pylori was detected in 28.43 of patients. We found that the vacAs1, vacAm2, and cagA genes with mean prevalences of 82.41, 71.42, and 69.23, respectively, were dominant in Iranian and Turkish patients. Conclusion: In the south of Turkey and northwest of Iran the studied genes were homogeneous and there were no significant differences in bacterial genetics. The results of this study indicate that cagA and vacAs1 are dominant genes in people with gastric disorders in our selected geographical regions. © TÜBİTAK.

Item Type: Article
Additional Information: Export Date: 16 February 2020 CODEN: TJMEE Correspondence Address: Bahadori, A.; Department of Medical Microbiology, Faculty of Medicine, Çukurova UniversityTurkey; email: dralibahadori@Yahoo.com
Uncontrolled Keywords: Adenocarcinoma CagPAI Chronic gastritis Helicobacter pylori VacA Article cagPAI gene DNA determination DNA extraction gene gene sequence genetic variability glmM gene Helicobacter infection human Iranian people major clinical study polymerase chain reaction spectrophotometry stomach adenocarcinoma stomach biopsy Turk (people) vacAm2 gene vacAs1 gene comparative study complication cross-sectional study gastritis genetics genotype genotyping technique Iran microbiology pathogenicity pathology stomach stomach tumor turkey (bird) bacterial protein VacA protein, Helicobacter pylori virulence factor Bacterial Proteins Cross-Sectional Studies Genotyping Techniques Helicobacter Infections Humans Stomach Neoplasms Turkey Virulence Factors
Subjects: WI Digestive System
QU Biochemistry
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 18 May 2020 05:18
Last Modified: 18 May 2020 05:18
URI: http://eprints.mums.ac.ir/id/eprint/16767

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