Evaluation of sensitivity of molecular methods for detection of rifampin-resistant strains amongst drug-resistant mycobacterium tuberculosis isolates

Behdani, M. and Rafatpanah, H. and Mohammadzadeh, A. and Ghazvini, K. and Amiri, M. and Mardaneh, J. (2017) Evaluation of sensitivity of molecular methods for detection of rifampin-resistant strains amongst drug-resistant mycobacterium tuberculosis isolates. Archives of Pediatric Infectious Diseases, 5 (3).

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Abstract

Background: Resistance to rifampin in multidrug-resistant M. tuberculosis (MDR-TB) is one of the major threats to the global public health system. Objectives: The aim of the present study was to explore the molecular characterization of resistance against rifampin amongst multidrug-resistant (MDR) and extensively drug-resistant (XDR) isolates obtained from patients. Methods: In this study, we used XDR (n = 6), MDR (n = 9) and rifampin sensitive (n = 39) isolates whose drug susceptibility patterns were identified by proportion method. A simple single-step multiplex-allele specific-polymerase chain reaction (MAS-PCR) assay was optimized to detect the three most frequent mutations in rifampin resistance-determining region (RRDR) fragment of rpoB gene. Additionally, single-strand conformational polymorphism (SSCP)-PCR and Sequencing were utilized. Results: Out of nine MDR isolates, five were detected by MAS-PCR as rifampin resistant (sensitivity; 55.5). In comparison with the proportion method, the sensitivity of SSCP for XDR, MDR and rifampin sensitive isolates were 83.3, 77.7 and 97.4, respectively. The DNA sequencing revealed that three of the 6 XDR isolates had several silent mutations, one isolate had one silent mutation, one strain had no mutations and only one isolate had mutations at codon 531. Conclusions: In sum, although the molecular methods are rapid, they are not able to identify resistance against rifampin efficiently. © 2016, Pediartric Infections Research Center.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Mohammadzadeh, A.; Microbiology Department, School of Medicine, Gonabad University of Medical SciencesIran; email: alm13604@gmail.com
Uncontrolled Keywords: Extensively drug-resistant Multidrug-resistant Mycobacterium tuberculosis Rifampin amikacin aminosalicylic acid capreomycin ciprofloxacin cycloserine ethambutol ethionamide genomic DNA isoniazid plus rifampicin kanamycin rifampicin RNA polymerase beta subunit streptomycin antibiotic resistance Article bacterial strain bacterium detection bacterium isolate clinical article codon controlled study cross-sectional study DNA sequence human multidrug resistant tuberculosis mutation nonhuman polymerase chain reaction silent mutation single strand conformation polymorphism
Subjects: WC Communicable Diseases
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 13 May 2020 07:20
Last Modified: 13 May 2020 07:20
URI: http://eprints.mums.ac.ir/id/eprint/16780

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