Effects of crocin on spatial memory impairment induced by hyoscine and its effects on bdnf, creb, and p-creb protein and mrna levels in rat hippocampus

Behravanfar, N. and Abnous, K. and Razavi, B. M. and Hosseinzadeh, H. (2017) Effects of crocin on spatial memory impairment induced by hyoscine and its effects on bdnf, creb, and p-creb protein and mrna levels in rat hippocampus. Jundishapur Journal of Natural Pharmaceutical Products, 12.

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Effects of crocin on spatial memory impairment induced by hyoscine and its effects on bdnf, creb, and p-creb protein and mrna levels in rat hippocampus.pdf

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Abstract

Background: Crocus sativus L., (Iridaceace), known as saffron, is used in traditional medicine for different purposes such asmemory improvment. Crocin is one of the pharmacologically active constituents responsible for many medicinal properties of saffron. Objectives: In this study, the effects of crocin, an active saffron constituent, on hyoscine inducedmemory impairment and its effect onprotein andmRNAtranscript levels of CREB (cAMP response element binding protein), BDNF(brain-derived neurotrophic factor), and phospho-CREB (p-CREB) were investigated in rat hippocampus. Methods: Rats were intraperitoneally treated with crocin (10, 20, and 40 mg/kg), normal saline (1 mL/kg), and rivastigmine (1.5 mg/kg) 30 minutes prior to hyoscine (1 mg/kg) for five days. The effects were studied on spacial learning and memory using Morris water maze. Protein and transcript levels of CREB and BDNF were analyzed using western blotting and qRT PCR methods, respectively. Results: Five days treatment with crocin (20 and 40 mg/kg) and rivastigmine significantly increased the presence of animals in target quadrant in probe trial on the 8th day compared to hyoscine. Crocin (20 mg/Kg) increased significantly BDNF and CREB protein and transcript levels compared to hyoscine. Conclusions: Crocin improved memory impairment induced by hyoscine through reduction of the latency to find the platform in the probe trial in Morris water maze test. Crocin improved memory probably due to the increase of BDNF and CREB protein and transcript levels. © 2017, Jundishapur Journal of Natural Pharmaceutical Products.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Hosseinzadeh, H.; Pharmaceutical Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical SciencesIran; email: hosseinzadehh@mums.ac.ir
Uncontrolled Keywords: BDNF CREB Crocin Morris water maze test p-CREB Spatial memory brain derived neurotrophic factor brain protein cyclic AMP responsive element binding protein messenger RNA rivastigmine scopolamine sodium chloride adult animal experiment animal model animal tissue Article controlled study down regulation drug efficacy drug isolation hippocampus latent period male neuroprotection nonhuman protein analysis quantitative analysis rat real time polymerase chain reaction RNA transcription scopolamine-induced amnesia spatial learning upregulation Western blotting
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 13 May 2020 07:33
Last Modified: 13 May 2020 07:33
URI: http://eprints.mums.ac.ir/id/eprint/16781

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