Relaxant effect of curcuma longa on rat tracheal smooth muscle and its possible mechanisms

Emami, B. and Shakeri, F. and Ghorani, V. and Boskabady, M. H. (2017) Relaxant effect of curcuma longa on rat tracheal smooth muscle and its possible mechanisms. Pharmaceutical Biology, 55 (1). pp. 2248-2258.

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Abstract

CONTEXT: Turmeric is a spice obtained from the root of Curcuma longa L. (Zingiberaceae) with anti-aging, anticancer, anti-Alzheimer's disease, antioxidant and other medicinal properties. OBJECTIVE: The relaxant effect of C. longa on rat tracheal smooth muscle and its possible mechanisms were investigated in this study. MATERIALS AND METHODS: The relaxant effects of four cumulative concentrations of hydro-ethanol extract of C. longa (6.25, 12.5, 25, 50 mg/mL) were studied on tracheal smooth muscle precontracted by methacholine or KCl in non-incubated or incubated with different substances including propranolol, diltiazem, L-NAME, glibenclamide, atropine, chlorpheniramine, indomethacin and papaverine. The duration of the study was 84 days. RESULTS: In non-incubated tracheal smooth muscle, the extract of C. longa showed significant concentration-dependent relaxant effects (p < 0.001 for all concentrations on both KCl and methacholine-induced contraction). There was no significant difference in the relaxant effects between C. longa and theophylline in both methacholine and KCl-induced contraction conditions. In tissues incubated with propranolol, diltiazem, L-NAME and glibenclamide on methacholine-induced contraction and in tissues incubated with atropine, chlorpheniramine, indomethacin and papaverine on KCl-induced contraction, the extract also showed significant concentration-dependent relaxant effects (p < 0.001). EC50 values of C. longa between non-incubated (16.22 ± 0.62) and incubated tissues (atropine: 13.03 ± 0.55, chlorpheniramine: 12.94 ± 0.68, indomethacin: 14.80 ± 0.57 and papaverine: 16.16 ± 1.42) were not significantly different. CONCLUSIONS: Tracheal smooth muscle relaxant effects of C. longa, were comparable to those of theophylline, which could be due to the presence of methylxanthines or its possible interaction with non-adrenergic non-cholinergic nervous system. © 2017 The Author(s).

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: PHBIF Correspondence Address: Boskabady, M.H.; Neurogenic Inflammation Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical SciencesIran; email: boskabadymh@mums.ac.ir
Uncontrolled Keywords: Beta adrenergic stimulation Calcium channel blocking Muscarinic receptor inhibition alcohol bronchodilating agent methacholine chloride plant extract potassium chloride theophylline animal chemistry comparative study Curcuma dose response drug effect male metabolism muscle contraction muscle relaxation plant root rat smooth muscle trachea Wistar rat Animals Bronchodilator Agents Dose-Response Relationship, Drug Ethanol Muscle, Smooth Plant Extracts Plant Roots Rats Rats, Wistar
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 12 May 2020 08:19
Last Modified: 12 May 2020 08:19
URI: http://eprints.mums.ac.ir/id/eprint/16806

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