Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30

Izadpanah, M. H. and Abbaszadegan, M. R. and Fahim, Y. and Forghanifard, M. M. (2017) Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30. Cellular and Molecular Biology Letters, 22 (1).

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Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30.pdf

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Abstract

Background: The transcription factor TWIST1 plays an important role in the epithelial-mesenchymal transition (EMT) process and in the migration, invasion and metastasis of cancer cells. OCT4, which is a homeobox transcription factor, has an important role in the self-renewal potential of cancer cells. Our aim here is to elucidate impact of ectopic expression of TWIST1 on OCT4 gene expression in esophageal squamous cell carcinoma (ESCC). Methods: The ESCC line was KYSE30. GP293T cells were transfected with purf-IRES-GFP and pGP plasmids to produce recombinant viral particles. A semi-confluent KYSE30 culture was transduced with the prepared retroviral particles. mRNA extraction and cDNA synthesis were performed from normal KYSE30 cells and those ectopically expressing TWIST1. Expressional analysis of TWIST1 and OCT4 were performed with relative comparative real-time PCR. Results: Ectopic expression of TWIST1 in KYSE30 cells was related to its significant overexpression: nearly nine-fold higher in GFP-hTWIST1 KYSE-30 cells than in control GFP cells. This induced expression of TWIST1 caused significant upregulation of OCT4 in GFP-hTWIST1 KYSE-30 cells: nearly eight-fold higher. In silico analysis predicted the correlation of TWIST1 and OCT4 through ETS2. Conclusions: Overexpressed TWIST1 can be correlated with upregulation of the cancer stem cell marker OCT4 and the protein may play critical regulatory role in OCT4 gene expression. Since OCT4 is involved in the self-renewal process, the results may suggest a new linkage between TWIST1 and OCT4 in the cell biology of ESCC, highlighting the probable role of TWIST1 in inducing self-renewal. © 2017 The Author(s).

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: CMBLF Correspondence Address: Forghanifard, M.M.; Mashhad University of Medical Sciences, Division of Human Genetics, Immunology Research Center, Avicenna Research InstituteIran; email: forghanifard@gmail.com
Uncontrolled Keywords: Cancer stem cells (CSCs) Esophageal squamous cell carcinoma (ESCC) OCT4 TWIST1 octamer transcription factor 4 Twist related protein 1 ETS2 protein, human nuclear protein POU5F1 protein, human transcription factor Ets 2 tumor marker TWIST1 protein, human Article carcinogenesis cell self-renewal controlled study esophageal squamous cell carcinoma ETS2 gene gene gene expression regulation gene interaction gene sequence genetic association KYSE-30 cell line OCT4 gene promoter region protein protein interaction sequence analysis transcription initiation site transcription regulation TWIST1 gene upregulation cancer stem cell ectopic expression esophagus tumor genetics human metabolism squamous cell carcinoma tumor cell line Biomarkers, Tumor Carcinoma, Squamous Cell Cell Line, Tumor Ectopic Gene Expression Esophageal Neoplasms Gene Expression Regulation, Neoplastic Humans Neoplastic Stem Cells Nuclear Proteins Octamer Transcription Factor-3 Promoter Regions, Genetic Proto-Oncogene Protein c-ets-2 Twist-Related Protein 1 Up-Regulation
Subjects: QU Biochemistry
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 27 Apr 2020 08:22
Last Modified: 27 Apr 2020 08:22
URI: http://eprints.mums.ac.ir/id/eprint/16865

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