Isolation and characterization of a chitionolytic enzyme producing microorganism, Paenibacillus chitinolyticus JK2 from Iran

Jami Al Ahmadi, K. and Tabatabaei Yazdi, M. and Fathi Najafi, M. and Shahverdi, A. R. and Faramarzi, M. A. and Zarrini, Gh and Behravan, J. (2008) Isolation and characterization of a chitionolytic enzyme producing microorganism, Paenibacillus chitinolyticus JK2 from Iran. Research Journal of Microbiology, 3 (6). pp. 395-404.

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Abstract

Chitinases are glycosyl hydrolases, which catalyze the degradation of chitin. These enzymes are capable of hydrolyzing chitin to its oligomers and monomer, N-acetyl-β-D-glucosamine. Fifty different chitin-degrading microorganisms were isolated in this study. One of these strains with high ability to produce chitinase was selected and identified as Paenibacillus chitinolyticus by morphological and biochemical properties along with 16S rDNA partial gene sequence analysis. This strain was able to produce high levels of extracellular chitinase in media containing chitin as sole carbon source. The chitinolylic activity of culture supernatant was maximal after 72 h of culture. The enzyme showed optimal activity at 37°C and a double optimum pH at pH 5 and 7. Chitooligosaccharides were the predominant products throughout the enzymatic hydrolysis of colloidal chitin, indicating that the enzyme was an endochitinase. This enzyme with these properties could be useful for waste treatment, chitooligosaccharides production and other relevant applications. © 2008 Academic Journals Inc.

Item Type: Article
Additional Information: Cited By :15 Export Date: 16 February 2020 Correspondence Address: Behravan, J.; Biotechnology Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, P.O. Box 91775-1365, Mashhad, Iran
Uncontrolled Keywords: Chitooligosaccharide Endochitinase Faenibacillus chirinolyticus Paenibacillus Paenibacillus chitinolyticus
Subjects: QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 19 May 2020 08:54
Last Modified: 23 May 2020 06:03
URI: http://eprints.mums.ac.ir/id/eprint/16874

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