Decreased Levels of Spleen Tissue CD4+CD25+Foxp3+ Regulatory T Lymphocytes in Mice Exposed to Berberine

Karimi, G. and Mahmoudi, M. and Balali-Mood, M. and Rahnama, M. and Zamani Taghizadeh Rabe, S. and Tabasi, N. and Riahi-Zanjani, B. (2017) Decreased Levels of Spleen Tissue CD4+CD25+Foxp3+ Regulatory T Lymphocytes in Mice Exposed to Berberine. JAMS Journal of Acupuncture and Meridian Studies, 10 (2). pp. 109-113.

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Abstract

The effects of isoquinoline alkaloid berberine (BER) on spleen tissue CD4+CD25+Foxp3+ regulatory T (Treg) cells were evaluated in BALB/c mice. Here, BER was administered daily by intraperitoneal injection at doses of 5 mg/kg and 10 mg/kg for 14 days. Following the exposure, mice spleen cellularities, IL-10 production by splenocytes, and spleen Treg/CD4+ cell profiles were studied in all the test groups of animals. The results showed that a high dose of BER (10 mg/kg) could decrease both the absolute and relative percentages of spleen Treg cells as well as decrease the production of IL-10 by splenocytes in the treated mice (p < 0.05). BER at 5 mg/kg did not appear to affect any of these parameters. Based on the finding here, it would seem that BER has effective immunostimulatory properties, which contradicts the results from other studies indicating immunosuppressive effects of BER. Depending on the doses of BER used, it might have a broad spectrum from immunosuppressive to stimulatory effects. Further studies, including more doses, are required to better evaluate the effects of this natural product. Mechanistic studies are required, particularly in case of redox state of the immune cells, to elucidate and determine how BER functions to impart the toxicity effects demonstrated here and in other studies. © 2017 Medical Association of Pharmacopuncture Institute

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Riahi-Zanjani, B.; Medical Toxicology Research Center, School of Medicine, Mashhad University of Medical SciencesIran; email: riahib@mums.ac.ir
Uncontrolled Keywords: BALB/c mice berberine spleen Treg cells cyclophosphamide interleukin 10 transcription factor FOXP3 CD4 antigen forkhead transcription factor Foxp3 protein, mouse Il2ra protein, mouse immunological adjuvant interleukin 2 receptor alpha animal cell animal experiment animal tissue Article CD4+ CD25+ T lymphocyte controlled study cytokine production drug dose comparison drug effect drug exposure drug mechanism drug megadose immunostimulation in vitro study in vivo study male mouse nonhuman oxidation reduction state priority journal regulatory T lymphocyte spleen cell spleen tissue animal Bagg albino mouse cytology drug effects immunology intraperitoneal drug administration randomization Adjuvants, Immunologic Animals Antigens, CD4 Forkhead Transcription Factors Injections, Intraperitoneal Interleukin-2 Receptor alpha Subunit Mice Mice, Inbred BALB C Random Allocation T-Lymphocytes, Regulatory
Subjects: QT physiology
QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 27 Apr 2020 07:33
Last Modified: 27 Apr 2020 07:33
URI: http://eprints.mums.ac.ir/id/eprint/16885

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