Quercetin induces apoptosis and necroptosis in MCF-7 breast cancer cells

Khorsandi, L. and Orazizadeh, M. and Niazvand, F. and Abbaspour, M. R. and Mansouri, E. and Khodadadi, A. (2017) Quercetin induces apoptosis and necroptosis in MCF-7 breast cancer cells. Bratislava Medical Journal, 118 (2). pp. 123-128.

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Abstract

OBJECTIVE: This study investigated the quercetin (Que) effects on growth of MCF-7 human cancer breast cell line and its cellular death mechanism. BACKGROUND: Quercetin has been found to be very efficacious against many different types of cancer cells. However, the study is not sufficiently powered to demonastrate anticancer mechanisms. METHODS: MCF-7cells were treated by 50 μM/ ml of Que for 48 hours. MCF-7 cells were also pretreated with 10 ?m ZVAD (apoptosis inhibitor) or 3 mM Nec-1 (necroptosis inhibitor) for evaluation of cell death induced by apoptosis or necroptosis. RESULTS: MTT and clonogenicity assays revealed that the Que induced a significant increase in cell viability and proliferation in presence of Nec-1 in comparison to the presence of ZVAD (p < 0.05). Que also increased apoptosis as revealed by DAPI staining and morphology evaluations. Following Que treatment Bcl-2 expression was significantly decreased while Bax expression was significantly increased. Que in presence of Nec-1 decreased expression of Bax gene, reduced apoptotic index, increased cell viability and proliferation of MCF-7 cells in comparison to absence of Nec-1. MCF-7 cells showed a significantly increased expression of RIPK1 and RIPK3 in response to Que plus ZVAD in comparison to absence of ZVAD. CONCLUSION: Our results revealed that the high Que toxicity for breast cancer cells depends on multiple cell death pathways, which involve mainly necroptosis.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 Correspondence Address: Niazvand, F.; Cell and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, P. O. Box 61335, Iran; email: niazvandf@gmail.com
Uncontrolled Keywords: Apoptosis Breast cancer Necroptosis Quercetin antioxidant receptor interacting protein serine threonine kinase RIPK1 protein, human RIPK3 protein, human breast tumor cell death cell survival drug effects female human MCF-7 cell line metabolism necrosis Antioxidants Breast Neoplasms Humans MCF-7 Cells Receptor-Interacting Protein Serine-Threonine Kinases
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib3 lib3
Date Deposited: 03 Mar 2020 10:36
Last Modified: 03 Mar 2020 10:36
URI: http://eprints.mums.ac.ir/id/eprint/16895

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