Effect of galbanic acid, a sesquiterpene coumarin from Ferula Szowitsiana, as an inhibitor of efflux mechanism in resistant clinical isolates of Staphylococcus Aureus

Bazzaz, B. S. F. and Memariani, Z. and Khashyarmanesha, Z. and Iranshahi, M. and Naderinasab, M. (2010) Effect of galbanic acid, a sesquiterpene coumarin from Ferula Szowitsiana, as an inhibitor of efflux mechanism in resistant clinical isolates of Staphylococcus Aureus. Brazilian Journal of Microbiology, 41 (3). pp. 574-580.

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Abstract

Galbanic acid, a sesquiterpene coumarin from Ferula szowitsiana roots, was investigated for its potentiating effect on the antimicrobial activity of antibiotics as well as ethidium bromide, in 6 multidrug resistance (MDR) clinical isolates of Staphylococcus aureus. Galbanic acid had inhibitory effect on none of the isolated bacteria tested (up to 800μg/ml). The MIC range of ciprofloxacin, tetracycline and ethidium bromide, against all tested S. aureus were 10-80, 10-80 and 4-16 μg/ml, respectively. These were reduced to ≤2.5-5, 2.5-5 and 0.5-2 μg/ml in the presence of galbanic acid (300μg/ml) or verapamil (100/ml). The rate of ethidium bromide (2 μg/ml) accumulation in clinical isolates was enhanced with galbanic acid (300μg/ml). There is also a decrease in loss of ethidium bromide from bacteria in the presence of galbanic acid. Similar results were obtained when verapamil (100 μg/ml) was used as an efflux pump inhibitor. Galbanic acid, like verapamil, a typical inhibitor of efflux pump, reduced the MIC of ethidium bromide and tested antibiotics. Since efflux is the only known reported mechanism for ethidium bromide resistance, the reduction in ethidium bromide MIC and enhanced accumulation as well as decreased efflux of ethidium bromide in the presence of galbanic acid, can be attributed to this efflux inhibitory properties.

Item Type: Article
Additional Information: Cited By :16 Export Date: 16 February 2020 Correspondence Address: Bazzaz, B. S. F.; School of Pharmacy and Biotechnology Research Center, Mashhad University of Medical Sciences (MUMS)Iran; email: fazlis@mums.ac.ir
Uncontrolled Keywords: Efflux pump inhibitor Ferula szowitsiana Galbanic acid Staphylococcus aureus Bacteria (microorganisms) Ferula Foeniculum vulgare
Subjects: QV pharmacology
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 23 Apr 2020 19:08
Last Modified: 23 Apr 2020 19:08
URI: http://eprints.mums.ac.ir/id/eprint/16941

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