Protective Effects of Aqueous and Ethanolic Extracts of Portulaca oleracea L. Aerial Parts on H 2O 2- Induced DNA Damage in Lymphocytes by Comet Assay

Behravan, J. and Mosafa, F. and Soudmand, N. and Taghiabadi, E. and Razavi, B. M. and Karimi, G. (2011) Protective Effects of Aqueous and Ethanolic Extracts of Portulaca oleracea L. Aerial Parts on H 2O 2- Induced DNA Damage in Lymphocytes by Comet Assay. JAMS Journal of Acupuncture and Meridian Studies, 4 (3). pp. 193-197.

[img] Text
Protective Effects of Aqueous and Ethanolic Extracts of Portulaca oleracea L. Aerial Parts on H2O2- Induced DNA Damage in Lymphocytes by Comet Assay.pdf

Download (469kB)
[img] Text
S2005290111000094

Download (62kB)

Abstract

The comet assay is a standard method for measuring DNA damage. In this study, the protective effects of ethanolic and aqueous extracts of Portulaca oleracea L. (P. oleracea) on human lymphocyte DNA lesions were evaluated with the comet assay. Lymphocytes were isolated from blood samples taken from healthy volunteers. Human lymphocytes were incubated in H 2O 2 (50,100, and 200μM), aqueous extract (0.05, 0.1, 0.5, 1, and 2.5mg/ml), and ethanolic extracts (0.05, 0.1, 0.5, 1, and 2.5mg/ml) of P. oleraceae aerial parts alone with a combination of H 2O 2 (100μM) with either 1 or 2.5mg/ml of both extracts at 4°C for 30 minutes. The extent of DNA migration was measured using the alkaline single cell gel electrophoresis approach assay, and DNA damage was expressed as percentage tail DNA. We found that the aqueous extract of P. oleracea significantly inhibited DNA damage, while there was no effect of the ethanolic extract. These data suggest that the aqueous extract of P. oleracea can prevent oxidative DNA damage to human lymphocytes, which is likely due to antioxidant constituents in the extract. © 2011.

Item Type: Article
Additional Information: Cited By :34 Export Date: 16 February 2020 Correspondence Address: Karimi, G.; Medical Toxicology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, Iran; email: karimig@mums.ac.ir
Uncontrolled Keywords: Antigenotoxicity Comet assay DNA damage Oxidative stress Portulaca oleracea Single-cell gel electrophoresis alcohol cell DNA hydrogen peroxide Portulaca oleracea extract alkalinity antioxidant activity aqueous solution article blood sampling cell isolation controlled study human human cell lymphocyte normal human priority journal Adult Antioxidants DNA Humans Lymphocytes Phytotherapy Plant Components, Aerial Plant Extracts Portulaca
Subjects: QV pharmacology
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 02 May 2020 09:33
Last Modified: 02 May 2020 09:33
URI: http://eprints.mums.ac.ir/id/eprint/16943

Actions (login required)

View Item View Item