Detection of Echinococcus multilocularis in carnivores in Razavi Khorasan Province, Iran using mitochondrial DNA

Beiromvand, M. and Akhlaghi, L. and Massom, S. H. and Mobedi, I. and Meamar, A. R. and Oormazdi, H. and Motevalian, A. and Razmjou, E. (2011) Detection of Echinococcus multilocularis in carnivores in Razavi Khorasan Province, Iran using mitochondrial DNA. PLoS Neglected Tropical Diseases, 5 (11).

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Detection of Echinococcus multilocularis in carnivores in Razavi Khorasan Province, Iran using mitochondrial DNA.PDF

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Abstract

Background: Echinococcus multilocularis is the source of alveolar echinococcosis, a potentially fatal zoonotic disease. This investigation assessed the presence of E. multilocularis infection in definitive hosts in the Chenaran region of Razavi Khorasan Province, northeastern Iran. Methodology/Principal Findings: Fecal samples from 77 domestic and stray dogs and 14 wild carnivores were examined using the flotation/sieving method followed by multiplex PCR of mitochondrial genes. The intestinal scraping technique (IST) and the sedimentation and counting technique (SCT) revealed adult Echinococcus in the intestines of five of 10 jackals and of the single wolf examined. Three jackals were infected only with E. multilocularis but two, and the wolf, were infected with both E. multilocularis and E. granulosus. Multiplex PCR revealed E. multilocularis, E. granulosus, and Taenia spp. in 19, 24, and 28 fecal samples, respectively. Echinococcus multilocularis infection was detected in the feces of all wild carnivores sampled including nine jackals, three foxes, one wolf, one hyena, and five dogs (6.5). Echinococcus granulosus was found in the fecal samples of 16.9 of dogs, 66.7 of jackals, and all of the foxes, the wolf, and the hyena. The feces of 16 (21.8) dogs, 7 of 9 (77.8) jackals, and all three foxes, one wolf and one hyena were infected with Taenia spp. Conclusions/Significance: The prevalence of E. multilocularis in wild carnivores of rural areas of the Chenaran region is high, indicating that the life cycle is being maintained in northeastern Iran with the red fox, jackal, wolf, hyena, and dog as definitive hosts. © 2011 Beiromvand et al.

Item Type: Article
Additional Information: Cited By :37 Export Date: 16 February 2020 Correspondence Address: Razmjou, E.; Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; email: erazmjou@tums.ac.ir
Uncontrolled Keywords: mitochondrial DNA article carnivore controlled study dog echinococcosis Echinococcus granulosus Echinococcus multilocularis feces analysis fox gene host hyena intestine parasite Iran jackal multiplex polymerase chain reaction nad1 gene nonhuman nucleotide sequence parasite identification prevalence sedimentation wolf animal animal disease Carnivora chemistry DNA sequence feces genetics isolation and purification methodology mixed infection molecular genetics parasitology polymerase chain reaction Taenia Animals Coinfection DNA, Mitochondrial Molecular Sequence Data Sequence Analysis, DNA
Subjects: QX Parasitology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 24 Apr 2020 18:16
Last Modified: 24 Apr 2020 18:16
URI: http://eprints.mums.ac.ir/id/eprint/16944

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