The effect of Nigella sativa extract on tracheal responsiveness and lung inflammation in valbuminsensitized guinea pigs

Boskabady, M. H. and Keyhanmanesh, R. and Khamneh, S. and Ebrahimi, M. A. (2011) The effect of Nigella sativa extract on tracheal responsiveness and lung inflammation in valbuminsensitized guinea pigs. Clinics, 66 (5). pp. 879-887.

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Abstract

OBJECTIVE: To examine the preventive effect of a hydro-ethanolic extract of Nigella sativa on the tracheal responsiveness and white blood cell count in the lung lavage fluid of sensitized guinea pigs. METHODS: Three groups of guinea pigs sensitized to intraperitoneally injected and inhaled ovalbumin were given drinking water alone (group S), drinking water containing a low concentration of N. sativa extract (group S+LNS) or drinking water containing a high concentration of N. sativa extract (group S+HNS). The tracheal responses of control animals (group C) and the three groups of sensitized guinea pigs (n = 7 for all groups) to methacholine were measured by the assessment of the tracheal smooth muscle response to increasing concentrations of methacholine, and the effective concentration causing 50 of the maximum response (EC50) was determined. Tracheal responses to 0.1 ovalbumin and white blood cell counts in the lung lavage fluid were also examined. RESULTS: The tracheal response of the group S guinea pigs to both methacholine and ovalbumin was significantly higher than the response of the controls (p,0.01 for both cases). The tracheal responses of the S+LNS and S+HNS groups to both methacholine and ovalbumin were significantly decreased compared to those of the S group (p,0.05 to p,0.01). The total white blood cell and eosinophil counts in the lung lavage fluid of group S were significantly higher than those of group C (p,0.01). The white blood cell counts in both treated groups showed significant improvements (p,0.01 for both cases). CONCLUSIONS: These results demonstrate the preventive effect of the N. sativa extract on the tracheal response and lung inflammation in sensitized guinea pigs. © 2011 CLINICS.

Item Type: Article
Additional Information: Cited By :39 Export Date: 16 February 2020 Correspondence Address: Boskabady, M. H.; Dept. of Physiology and Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; email: boskabadymh@mums.ac.ir
Uncontrolled Keywords: Asthma Inflammation Nigella sativa Sensitization Tracheal responsiveness bronchodilating agent methacholine chloride ovalbumin plant extract animal article black cumin bronchus hyperreactivity cell count chemically induced disorder chemistry dose response drug effect guinea pig lung lavage male pathology pneumonia trachea Animals Bronchial Hyperreactivity Bronchoalveolar Lavage Bronchodilator Agents Dose-Response Relationship, Drug Guinea Pigs Plant Extracts
Subjects: WF Respiratory System
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 04 May 2020 04:40
Last Modified: 04 May 2020 04:40
URI: http://eprints.mums.ac.ir/id/eprint/16949

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