Inhibitory effect of Nigella sativa on histamine (H1) receptors of isolated guinea pig tracheal chains

Boskabady, M. H. and Sheiravi, N. (2002) Inhibitory effect of Nigella sativa on histamine (H1) receptors of isolated guinea pig tracheal chains. Pharmaceutical Biology, 40 (8). pp. 596-602.

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Abstract

In a previous study, the relaxant and anticholinergic (functional antagonism) effects of Nigella sativa have been demonstrated on guinea pig tracheal chains. To elucidate the other mechanisms responsible for this relaxant effect, the inhibitory effect of this plant on histamine H1 receptors was examined in this study. The antihistaminic effects of aqueous and macerated extracts, 5 nM chlorpheniramine, and saline were tested by performing the cumulative log concentration-response curves of histamine induced contraction of isolated guinea pig tracheal chains incubated with three different conditions including: 1) 1.4 μM indomethacin, 2) indomethacin, 1 μM propranolol, and 10nM atropine, and 3) indomethacin and propranolol (for each group n = 8). The results showed clear parallel rightward shifts in histamine-response curves obtained in the presence of macerated extract in group 1 and aqueous extract in group 2 experiments compared with the curves obtained in the presence of saline. The EC50 (effective concentration of histamine causing 50 of maximum response) obtained in the presence of extracts, and chlorpheniramine in all three sets of experiments were significantly higher than that of saline (P < 0.05 to p = 0.002), but maximum response to histamine obtained in the presence of extracts were lower (P < 0.01 to P < 0.001). However, the maximum response obtained in the presence of aqueous extract in group 2 experiments compared to the other two sets of experiments was improved. These results indicated a competitive antagonistic effect of Nigella sativa at histamine H1 receptors.

Item Type: Article
Additional Information: Cited By :30 Export Date: 16 February 2020 CODEN: PHBIF Correspondence Address: Boskabady, M.H.; Dept. of Physiology, Ghaem Medical Centre, Mashhad, Post Code 91735, Iran
Uncontrolled Keywords: Antihistaminic effect Guinea pig Nigella sativa Trachea atropine chlorpheniramine histamine histamine H1 receptor histamine H1 receptor antagonist indometacin Nigella sativa extract propranolol sodium chloride animal tissue anticholinergic effect aqueous solution article black cumin controlled study dose response drug isolation male nonhuman plant receptor blocking smooth muscle contraction smooth muscle relaxation Animalia Cavia porcellus Sus scrofa
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 29 Apr 2020 09:22
Last Modified: 29 Apr 2020 09:22
URI: http://eprints.mums.ac.ir/id/eprint/16953

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