Possible mechanism(s) for relaxant effect of aqueous and macerated extracts from Nigella sativa on tracheal chains of guinea pig

Boskabady, M. H. and Shirmohammadi, B. and Jandaghi, P. and Kiani, S. (2004) Possible mechanism(s) for relaxant effect of aqueous and macerated extracts from Nigella sativa on tracheal chains of guinea pig. BMC Pharmacology, 4.

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Abstract

Background: In previous studies, the relaxant, anticholinergic (functional antagonism) and antihistaminic effects of Nigella sativa have been demonstrated on guinea pig tracheal chains. To elucidate the other mechanisms responsible for the relaxant effect of this plant, its inhibitory effect on the calcium channel was examined in this study. Results: The inhibitory effects of both concentrations of diltiazem in all three groups of experiments were significantly greater than those of saline (p < 0.01 to P < 0.001). The inhibitory of two larger concentrations of aqueous extracts in group 1 and 2 were significantly greater than those of saline (p < 0.01 to P < 0.001). The effect of two larger concentrations of macerated extract in group 1 and all concentrations of this extract in group 2 were also significantly greater than those of saline (p < 0.01 to P < 0.001). However, the extract of Nigella sativa did not show any inhibitory effect in group 3. There was a significant correlation between inhibitory effect and increasing concentrations for both extracts and diltiazem in groups 1 and 2 (p < 0.05 to p < 0.005). Conclusion: Although the extracts of Nigella sativa showed inhibitory effects on pre-contracted tracheal chains in the presence of both ordinary and calcium free Krebs solution, the absence of inhibitory effects of the extracts on KCl induced contraction of tracheal chains suggest that the calcium channel blocking effect of this plant dose not contribute to the relaxant effect of this plant on the tracheal chains of guinea pigs. © 2004 Boskabady et al; licensee BioMed Central Ltd.

Item Type: Article
Additional Information: Cited By :55 Export Date: 16 February 2020 CODEN: BPMHB Correspondence Address: Boskabady, M.H.; Department of Physiology, Ghaem Medical Centre, Mashhad Univ. of Med. Sciences, Mashhad, Iran; email: mhboskabady@hotmail.com
Uncontrolled Keywords: Aqueous extract Calcium antagonistic effect Guinea pig Macerated extract Nigella sativa Trachea calcium calcium channel diltiazem Nigella sativa extract potassium chloride sodium chloride animal tissue aqueous solution article black cumin concentration response controlled study drug isolation drug mechanism male nonhuman statistical significance trachea obstruction Animals Bronchodilator Agents Calcium Channel Blockers Calcium Channels Dose-Response Relationship, Drug Guinea Pigs Muscle Relaxation Plant Extracts Plants, Medicinal Potassium Channels Water
Subjects: QT physiology
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 02 May 2020 04:48
Last Modified: 02 May 2020 04:48
URI: http://eprints.mums.ac.ir/id/eprint/16954

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