Efficacy of the antiadhesin octyl O-(2-acetamido-2-deoxy-β-D- galactopyranosyl)-(1-4)-2-O-propyl-β-D-galactopyranoside (Fimbrigal-P) in a rat oral candidiasis model

Foldvari, M. and Jaafari, M. R. and Radhi, J. and Segal, D. (2005) Efficacy of the antiadhesin octyl O-(2-acetamido-2-deoxy-β-D- galactopyranosyl)-(1-4)-2-O-propyl-β-D-galactopyranoside (Fimbrigal-P) in a rat oral candidiasis model. Antimicrobial Agents and Chemotherapy, 49 (7). pp. 2887-2894.

[img] Text
0527-04.pdf

Download (434kB)
[img] Text
Efficacy of the antiadhesin octyl O-(2-acetamido-2-deoxy-β-D- galactopyranosyl)-(1-4)-2-O-propyl-β-D-galactopyranoside (Fimbrigal-P) in a rat oral candidiasis model.pdf

Download (434kB)

Abstract

Adherence of Candida albicans to buccal epithelial cells via its fimbrial subunit requires the minimal disaccharide sequence β-GalNAc(1-4)-β- galactosidase in host cell receptors asialo-GM1 or asialo-GM2. This and other disaccharides and some of its synthetic derivatives have been shown to inhibit purified fimbrial or pathogen binding in vitro. This study evaluates the in vivo efficacy of the propyl derivative of this disaccharide, octyl O-(2-acetamido-2-deoxy-β-D-galactopyranosyl)-(1-4)-2-O-propyl-β-D- galactopyranoside, or Fimbrigal-P, incorporated into a mucoadhesive polymer formulation in a rat oral candidiasis model. Colony counts of microcurette samples from the oral cavity and tongue homogenates were used to estimate the effectiveness of four treatment modalities to reduce oral fungal burden. All treatment modalities (preventative, premixing with the Candida inoculant, drinking water, and treatment) significantly reduced fungal burden compared to untreated control animals by day 9; however, the preventative and premixing approaches provided a faster rate of fungal clearance. The low toxicity and immunogenicity of this synthetic carbohydrate and its stability in saliva, as demonstrated by high-performance liquid chromatography, make it a promising candidate for the prevention and treatment of microbial infections in which the pathogen relies on the β-GalNAc(1-4)-β-galactosidase disaccharide to establish adherence. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Item Type: Article
Additional Information: Cited By :5 Export Date: 16 February 2020 CODEN: AMACC Correspondence Address: Foldvari, M.; College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Sask. S7N 5C9, Canada; email: foldvari@duke.usask.ca
Uncontrolled Keywords: adhesin beta galactosidase carbohydrate carbomer carbopol ex 214 cell receptor disaccharide fimbrigal P ganglioside GM2 gangliotetraosylceramide gangliotriosylceramide n acetylgalactosamine polymer propane unclassified drug animal experiment animal model animal tissue article Candida albicans cell adhesion cheek mucosa controlled study drug efficacy drug formulation drug stability fimbria fungal colonization high performance liquid chromatography immune deficiency immunogenicity in vivo study mouth cavity nonhuman priority journal rat thrush tongue Animals Candidiasis, Oral Disaccharides Dose-Response Relationship, Drug Fungal Proteins Polymers Rats Rats, Sprague-Dawley Saliva Treatment Outcome
Subjects: QV pharmacology
QX Parasitology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 25 Apr 2020 08:41
Last Modified: 25 Apr 2020 08:41
URI: http://eprints.mums.ac.ir/id/eprint/16978

Actions (login required)

View Item View Item