Cancer-testis gene expression profiling in esophageal squamous cell carcinoma: Identification of specific tumor marker and potential targets for immunotherapy

Forghanifard, M. M. and Gholamin, M. and Farshchian, M. and Moaven, O. and Memar, B. and Forghani, M. N. and Dadkhah, E. and Naseh, H. and Moghbeli, M. and Raeisossadati, R. and Abbaszadegan, M. R. (2011) Cancer-testis gene expression profiling in esophageal squamous cell carcinoma: Identification of specific tumor marker and potential targets for immunotherapy. Cancer Biology and Therapy, 12 (3). pp. 191-197.

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Abstract

Cancer-testis antigens (CTAs) are often specifically expressed in cancer cells and under normal conditions are only considered to be expressed in the germ line cells and the placenta. CTAs are potential targets for cancer immunotherapy and therefore necessitates their expression profiling. The expression profile of LAGE1, MAGE-A4 and NY-ES O1, their possible correlations and interaction, and the clinicopathological associations of each marker were studied. RNA was extracted from fresh esophagectomy tissues of 41 esophageal squamous cell carcinoma (ES CC) patients prior to any other therapeutic intervention. The relative mRNA expression of LAGE1, MAGE-A4 and NY-ES O1 was assessed with the real-time reverse transcription-polymerase chain reaction (RT-PCR) 5′ nuclease assay. The overexpression of LAGE1, MAGE-A4 and NY-ES O1 was found in 39, 90.2 and 41.4 of ES CC samples respectively. Of the patients, 97.5 showed an overexpression of at least one CTA. The relative expression of MAGE-A4 was directly associated with lymph node metastasis and the stage of the tumor (p < 0.05). A significant direct correlation was also detected between the MAGE-A4/LAGE1 and MAGE-A4/NY-ES O1 levels of gene expression. MAGE-A4 is identified as a specific biomarker of ES CC with a possible oncogenic role contributing to tumor progression. Interactions between MAGE-A4, LAGE1 and NY-ES O1 and their significant clinical consequences introduce these CTAs as appropriate targets for a polyvalent cancer vaccine. © 2011 Landes Bioscience.

Item Type: Article
Additional Information: Cited By :34 Export Date: 16 February 2020 Correspondence Address: Forghanifard, M.M.; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; email: Forghanifard@gmail.com
Uncontrolled Keywords: Cancer testis antigens Cancer vaccine Esophageal squamous cell carcinoma Real-time PCR Tumor marker cancer testis antigen immunomodulating agent melanoma antigen 4 nuclease adult aged antigen expression article assay cancer cell cancer immunotherapy cancer staging clinical article controlled study correlation analysis esophagus resection female gene expression profiling gene interaction gene overexpression genetic association human human cell human tissue Lagenidium lymph node metastasis male pathology protein interaction real time polymerase chain reaction reverse transcription polymerase chain reaction RNA extraction tumor growth Aged, 80 and over Antigens, Neoplasm Carcinoma, Squamous Cell Esophageal Neoplasms Gene Expression Regulation, Neoplastic Humans Immunotherapy Lymphatic Metastasis Membrane Proteins Middle Aged Neoplasm Proteins Real-Time Polymerase Chain Reaction Testis Tumor Markers, Biological
Subjects: WI Digestive System
WJ Urogenital System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 20 Apr 2020 06:44
Last Modified: 20 Apr 2020 06:44
URI: http://eprints.mums.ac.ir/id/eprint/16979

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