Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: Preliminary step for DC vaccine design

Gholamin, M. and Moaven, O. and Farshchian, M. and Mahmoudi, M. and Sankian, M. and Memar, B. and Forghani, M. N. and Malekzadeh, R. and Rajabi-Mashhadi, M. T. and Abbaszadegan, M. R. (2010) Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma: Preliminary step for DC vaccine design. BMC Cancer, 10.

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Induction of cytotoxic T lymphocytes primed with Tumor RNA-loaded Dendritic Cells in esophageal squamous cell carcinoma.pdf

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Abstract

Background: Dendritic Cells (DC) are potent antigen presenting cells with the ability to prime naïve T cells and convert them to cytotoxic T-lymphocytes (CTL). We evaluated the capability of autologous DCs transfected with total tumor and normal RNA to induce cytotoxic CTL as the preliminary step to design a DC-based vaccine in the esophageal squamous cell carcinoma (ESCC).Methods: Monocytes-derived DCs were electroporated with either total tumor RNA or normal RNA. T cells were then primed with tumor RNA transfected DCs and lytic effects of the generated CTL were measured with Cytotoxicity assay and IFN-γ Release Elispot assay.Results: Cytotoxicity was induced against DCs loaded with tumoral RNA (24.8 ± 5.2 SEM) while in normal RNA-loaded DCs, it was minimal (6.1 ± 2.4 SEM) and significantly lower (p < 0.05). INF-γ secretion was more than 2-folds higher in tumoral RNA-loaded DCs when compared with normal RNA-loaded DCs (p < 0.05).Conclusion: Electroporating DCs with tumor RNA generated tumor antigen presenting cells which in turn enhanced cytotoxic effects of the T cells against ESCC. This may be a useful autologous ex vivo screening tool for confirming the lytic effects of primed T cells on tumors and evaluate probable further adverse effects on noncancerous tissues. These data provide crucial preliminary information to establish a total tumor RNA-pulsed DC vaccine therapy of ESCC. © 2010 Gholamin et al; licensee BioMed Central Ltd.

Item Type: Article
Additional Information: Cited By :19 Export Date: 16 February 2020 CODEN: BCMAC Correspondence Address: Abbaszadegan, M.R.; Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran; email: abbaszadeganmr@mums.ac.ir
Uncontrolled Keywords: dendritic cell vaccine gamma interferon green fluorescent protein messenger RNA tumor antigen cancer vaccine adult aged antigen presenting cell article case report controlled study cytokine release cytotoxic T lymphocyte cytotoxicity dendritic cell drug design electroporation enzyme linked immunospot assay esophagus carcinoma female fluorescence activated cell sorting genetic transfection human human cell human tissue in vitro study male monocyte RNA isolation squamous cell carcinoma autotransplantation cell culture cell separation cytotoxicity test esophagus tumor flow cytometry genetics immunology immunophenotyping lymphocyte activation middle aged transplantation Aged, 80 and over Cancer Vaccines Carcinoma, Squamous Cell Cells, Cultured Cytotoxicity Tests, Immunologic Cytotoxicity, Immunologic Dendritic Cells Esophageal Neoplasms Humans Interferon-gamma RNA, Messenger T-Lymphocytes, Cytotoxic Transfection Transplantation, Autologous
Subjects: WI Digestive System
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 29 Apr 2020 08:54
Last Modified: 29 Apr 2020 08:54
URI: http://eprints.mums.ac.ir/id/eprint/16993

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