Modified polyethyleneimine with histidine-lysine short peptides as gene carrier

Hashemi, M. and Parhiz, B. H. and Hatefi, A. and Ramezani, M. (2011) Modified polyethyleneimine with histidine-lysine short peptides as gene carrier. Cancer Gene Therapy, 18 (1). pp. 12-19.

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Abstract

There are several strategies that can be utilized to improve transfection efficiency while reducing the cytotoxicity of polyethyleneimine (PEI) as a promising non-viral gene delivery vector. In this study, we evaluated the potential use of lysine-histidine (KH) peptides in modifying the PEI 10 kDa structure and enhancing its efficiency while maintaining low toxicity of PEI. PEI 10 kDa was modified with 6-bromohexanoic acid (alkyl) to increase its lipophilicity. Then, ethylenediamine (EDA) was attached to the carboxylic groups of PEI-hexanoate to restore the primary amines of PEI. Subsequently, six different KH short peptides were conjugated to PEIs and evaluated for the effect of the KH sequence on vector transfection efficiency and cytotoxicity. The transfection efficiency of PEI-peptides complexed with a luciferase reporter gene (pRLCMV) in Neuro-2A murine neuroblastoma cells showed that the PEI conjugated to KHHHKKHHHK peptide had a significantly higher rate of gene transfection efficiency in comparison with other KH peptides. This peptide was conjugated to PEI-alkyl and PEI-alkyl-EDA and significant improvement in efficiency with minimal cytotoxicity was observed. The results obtained suggest that the sequence and content of KH peptides will have a significant impact on the transfection efficiency of modified PEI 10 kDa. © 2011 Nature America, Inc. All rights reserved.

Item Type: Article
Additional Information: Cited By :33 Export Date: 16 February 2020 CODEN: CGTHE Correspondence Address: Ramezani, M.; Pharmaceutical Research Center, School of Pharmacy, P.O. Box: 91775-1365, Mashhad, Iran; email: ramezanim@mums.ac.ir
Uncontrolled Keywords: histidine lysine non-viral vector polyethyleneimine alkyl group amine carboxyl group edoxudine ethylenediamine gestonorone caproate hexanoic acid luciferase peptide amino acid sequence animal cell article clinical evaluation controlled study drug conjugation drug cytotoxicity drug effect drug efficacy drug structure drug toxicity drug use genetic transfection heterozygote lipophilicity neuroblastoma cell nonhuman priority journal protein modification reporter gene Animals Cell Line, Tumor Cell Survival Cells, Cultured Genetic Vectors Humans Mice Peptides Transfection Murinae
Subjects: QU Biochemistry
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 30 Apr 2020 07:15
Last Modified: 30 Apr 2020 07:15
URI: http://eprints.mums.ac.ir/id/eprint/17001

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