Pattern of laminin expression during kidney morphogenesis in Balb/c mice

Houshang, R. and Reza, N. M. and Mehdi, J. (2010) Pattern of laminin expression during kidney morphogenesis in Balb/c mice. Pakistan Journal of Biological Sciences, 13 (19). pp. 961-965.

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Basement membrane of glomerular mesangium (BMG) is one of important components which play a key role to support of the capillary loops in a renal glomerulus and completeness of BMG due to interaction of ureteric bud and metanephric mesenchyme during glomerulogenesis. As laminin contribute in extra cellular matrix and especially in basement membrane, the aim of the present study was to demonstrate the distribution of this molecule so, in this investigation specific antibody against laminin have been used in light microscopy to study development of BMG of fetal and postnatal mouse glomerular mesangium. Female inbred Balb/c mice were selected and were kept under normal condition and finding vaginal plug was assumed as day zero of pregnancy. Two pregnant mice were sacrificed by cervical dislocation in one of gestational days 13-18, respectively and their fetuses were fixed, serially sectioned and by using antibody against laminin in BMG were carried out. The same process was used for kidneys preparation at 15 postnatal days. Present data revealed that laminin showed weak reaction on day 14 of gestation. The amount of laminin increased continuously until next days of fetal life and primary of 10 days postnatal in BMG. After this period, laminin reaction did not show significant change in newborns. These data indicate that laminin appears just during the glomerulogenesis and because of continuity with vasculature which is required for Extra Cellular Matrix (ECM) and glomerular endothelial cell differentiation, laminin, is the one of major structural proteins in BMG. © 2010 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Mehdi, J.; Department of Anatomy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Uncontrolled Keywords: Basement membrane Development Extra cellular matrix Mus
Subjects: QS Human Anatomy
WJ Urogenital System
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 02 May 2020 04:10
Last Modified: 02 May 2020 04:10

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