Characterization and transdifferentiation of human mesothelial progenitor/stem cells of the peritoneum cavity

Jahangiri, L. and Moallem, S. A. and Foroutan, T. and Hosseini, A. and Nazemian, F. (2007) Characterization and transdifferentiation of human mesothelial progenitor/stem cells of the peritoneum cavity. Journal of Biological Sciences, 7 (7). pp. 1179-1186.

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Mesothelial progenitor cells have been reported to reside in either the monolayer of mesothelium, submesothelium or within the peritoneal cavity as free floating cells. A putative plasticity has been suggested for these cells as an epithelial to mesenchymal transition and transformation into myofibroblasts and smooth muscle have been suggested. In order to investigate the plasticity and nature of mesothelial cells, cell populations from peritoneal dialysis fluid of early stage non-peritonitis patients were first screened for dominant marker determination by RT-PCR and immunofluorescence. Then, cell colonies were isolated by culture and FACS using HBME-1 and CD34 markers. Efficacy of cell colony isolation by the mentioned methods was validated by flow cytometry. Later, specific media for the differentiation of the mesothelial colonies were defined. The culture of mesothelial cell colonies in knockout serum cultures containing specific growth factors showed a surprising but a relatively low yield of differentiation capacity along extra mesodermal lineage directed to neurons. This was evident by morphological characteristics of neurons and expression of neuronal specific cell markers consisting of the immature neuron markers Tubulin III and Nestin and also the structural neuronal marker Neurofilament 200 as revealed by Western blot. This study could completely violate the previously assumed plasticity of mesothelial progenitor cells and lead us to the definition of a new source of adult stem cells. © 2007 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Moallem, S.A.; School of Pharmacy, P.O. Box 91775-1365, Mashhad, Iran
Uncontrolled Keywords: CD34+ HBME-1 Peritoneum Stem cells Transdifferentiation CD31 antigen CD34 antigen CD38 antigen growth factor HBME 1 protein nestin neurofilament protein Thy 1 antigen tubulin tumor marker unclassified drug article cell culture cell differentiation cell isolation cell lineage cell population cell structure cell transformation controlled study culture medium epithelium cell flow cytometry fluorescence activated cell sorting human human cell immunocytochemistry immunofluorescence mesenchyme cell mesoderm mesothelium cell myofibroblast nerve cell peritoneal cavity peritoneal dialysis peritoneal fluid peritoneum cell plasticity protein expression reverse transcription polymerase chain reaction smooth muscle fiber stem cell validation study Western blotting
Subjects: WI Digestive System
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 20 Apr 2020 08:52
Last Modified: 20 Apr 2020 08:52

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