Decreased circulating level in parallel with lack of associated genetic variation in CXCL10 (IP-10) in southeastern post-transfusion occult HBV-infected patients

Karimabad, M. N. and Hassanshahi, G. and Arababadi, M. K. and Shabani, Z. and Shamsizadeh, A. and Rafatpanah, H. and Yaghini, N. and Shirdel, A. (2011) Decreased circulating level in parallel with lack of associated genetic variation in CXCL10 (IP-10) in southeastern post-transfusion occult HBV-infected patients. Laboratory Medicine, 42 (7). pp. 423-426.

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Decreased circulating level in parallel with lack of associated genetic variation in CXCL10 (IP-10) in southeastern post-transfusion occult HBV-infected patients.pdf

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Abstract

Background: The presence of hepatitis B virus (HBV) DNA in parallel with the absence of a measurable amount of hepatitis B surface antigen (HBsAg) in periphery of hepatitis B-contaminated carriers is characterized as occult hepatitis B infection (OBI). Its clinical status has resulted in multiple drawbacks for blood transfusion services worldwide. Therefore, the aim of the current study was to investigate the association between polymorphisms in -1443 region of C-X-C motif chemokine 10 (CXCL10) interferon-inducible protein-10 (IP-10) and its plasma level in patients with post-transfusion transmitted OBI. Material and Methods: In this experimental study, plasma samples from 3700 blood donors were tested for HBsAg and anti-hepatitis B core antibody (HBc) by enzyme-linked immunosorbent assay (ELISA). The HBsAg-/anti-HBc+ samples were selected and screened for HBV-DNA by polymerase chain reaction (PCR). Hepatitis B virus DNA-positive samples considered OBI cases, and a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to examine the polymorphisms in the CXCL10 (IP-10) gene. The plasma levels of CXCL10 (IP-10) were also detected using ELISA Results: The results of this study demonstrated that 352 (9.5) out of 3700 blood samples were HBsAg-/anti-HBc+, and HBV DNA was detected in 57/352 (16.1) of HBsAg-/anti-HBc+ samples. The results of this study also showed that the plasma level of IP-10 was 87.59 ± 8.75 and 143.5 ± 4.83 pg/mL in OBI patients and healthy controls, respectively. Statistical analysis showed the difference was significant (P<0.001). Our results also showed that all of the patients and healthy controls had AG genotypes, while other genotypes were not seen in patients and controls. Conclusion: According to the findings of this study, it can be concluded that OBI patients lack the ability to express adequate amounts of IP-10 that could probably affect the process of HBV clearance. © 2011 by The American Society for Clinical Pathology.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: LBMEB Correspondence Address: Shirdel, A.; Department of Internal Medicine, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran; email: ShirdelA@mums.ac.ir
Uncontrolled Keywords: Chemokine CXCL10 (IP-10) Occult hepatitis B infection Polymorphism gamma interferon inducible protein 10 hepatitis B antigen hepatitis B core antibody virus DNA adult article blood transfusion controlled study DNA polymorphism enzyme linked immunosorbent assay female genetic variability genotype hepatitis B human major clinical study male nonhuman occult blood polymerase chain reaction restriction fragment length polymorphism Hepatitis B virus
Subjects: WH Hemic and Lymphatic System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 05 May 2020 04:51
Last Modified: 05 May 2020 04:51
URI: http://eprints.mums.ac.ir/id/eprint/17028

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