Molecular characterization and RAPD analysis of Juniperus species from Iran

Kasaian, J. and Behravan, J. and Hassany, M. and Emami, S. A. and Shahriari, F. and Khayyat, M. H. (2011) Molecular characterization and RAPD analysis of Juniperus species from Iran. Genetics and molecular research : GMR, 10 (2). pp. 1069-1074.

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Abstract

The genus Juniperus L. (Cupressaceae), an aromatic evergreen plant, consists of up to 68 species around the world. We classified five species of Juniperus found in Iran using molecular markers to provide a means for molecular identification of Iranian species. Plants were collected (three samples of each species) from two different provinces of Iran (Golestan and East Azarbayejan). The DNA was extracted from the leaves using a Qiagen Dneasy Plant Mini Kit. Amplification was performed using 18 ten-mer RAPD primers. Genetic distances were estimated based on 187 RAPD bands to construct a dendrogram by means of unweighted pair group method of arithmetic means. It was found that J. communis and J. oblonga were differentiated from the other species. Genetic distance values ranged from 0.19 (J. communis and J. oblonga) to 0.68 (J. communis and J. excelsa). Juniperus foetidissima was found to be most similar to J. sabina. Juniperus excelsa subspecies excelsa and J. excelsa subspecies polycarpos formed a distinct group.

Item Type: Article
Additional Information: Cited By :5 Export Date: 16 February 2020 Correspondence Address: Kasaian, J.email: kasaeeianj1@mums.ac.ir
Uncontrolled Keywords: primer DNA article genetics Iran Juniperus nucleotide sequence random amplified polymorphic DNA Base Sequence DNA Primers Random Amplified Polymorphic DNA Technique
Subjects: QV pharmacology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 30 Apr 2020 07:25
Last Modified: 30 Apr 2020 07:25
URI: http://eprints.mums.ac.ir/id/eprint/17030

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