A rat model of early stage osteonecrosis induced by glucocorticoids

Kerachian, M. A. and Harvey, E. J. and Cournoyer, D. and Chow, T. Y. and Nahal, A. and Séguin, C. (2011) A rat model of early stage osteonecrosis induced by glucocorticoids. Journal of Orthopaedic Surgery and Research, 6 (1).

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Abstract

Background: Glucocorticoid (GC)-induced osteonecrosis (ON) is an important complication of medical therapy. The exact pathomechanisms of ON has not been clearly elucidated. There is a need for a reproducible animal model that better approximates the clinical scenario.Methods: To determine the genetic susceptibility of rats to develop GC-induced femoral head ON, we evaluated 5 different inbred strains of rats (Spontaneous Hypertensive Rat, Wistar Kyoto, Wistar Furth, SASCO Fisher and Lewis). Prednisone pellets (dosage of 1.82-2.56 mg/kg/day) were implanted subcutaneously for 90. After 90 days, the femurs were resected and examined histologically and radiographically. Pathological and histological examination was performed. Hematoxylin and eosin (H & E) staining was used to delineate the femoral head osteonecrosis lesions as well as abnormalities of articular cartilage and growth plate.Results: The greatest differences in H & E staining were seen in the Wistar Kyoto and Wistar Furth groups. In these groups 4 out of 5 and 3 out of 5, respectively, steroid-induced rats revealed growth plate disruption with acellular areas. The TUNEL apoptosis staining assay for apoptosis revealed that 4 out of 5 of Wistar Kyoto rats, 5 out of 5 of Wistar Furth, 2 out of 4 of surviving Lewis and 2 out of 2 of the surviving spontaneous hypertensive rats had apoptotic osteocytes in trabeculae, whereas none of the Fisher rats showed apoptotic osteocytes.Conclusions: We postulate that Wistar Kyoto, Wistar Furth and spontaneous hypertensive rats may be strains of rats more susceptible to develop ON of the femoral head while Fisher rats were the most resistant. © 2011 Kerachian et al; licensee BioMed Central Ltd.

Item Type: Article
Additional Information: Cited By :21 Export Date: 16 February 2020 Correspondence Address: Séguin, C.; Department of Medicine, Division of Haematology, McGill University Health Center (MUHC), 1650 Cedar Avenue, Montreal, QC H3G 1A4, Canada; email: chantal.seguin@muhc.mcgill.ca
Uncontrolled Keywords: glucocorticoid prednisone animal apoptosis article chemically induced disorder disease model drug effect drug implant female femur head necrosis genetic predisposition genetics growth plate osteocyte pathology pilot study rat species difference Animals Disease Models, Animal Drug Implants Genetic Predisposition to Disease Glucocorticoids Osteocytes Pilot Projects Rats Species Specificity
Subjects: WE Musculoskeletal system
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 05 May 2020 03:56
Last Modified: 05 May 2020 03:56
URI: http://eprints.mums.ac.ir/id/eprint/17031

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