Antibacterial efficacy of lytic bacteriophages against antibiotic-resistant Klebsiella species

Khajeh Karamoddini, M. and Fazli-Bazzaz, B. S. and Emamipour, F. and Sabouri Ghannad, M. and Jahanshahi, A. R. and Saed, N. and Sahebkar, A. (2011) Antibacterial efficacy of lytic bacteriophages against antibiotic-resistant Klebsiella species. TheScientificWorldJournal, 11. pp. 1332-1340.

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Bacterial resistance to antibiotics is a leading and highly prevalent problem in the treatment of infectious diseases. Bacteriophages (phages) appear to be effective and safe alternatives for the treatment of resistant infections because of their specificity for bacterial species and lack of infectivity in eukaryotic cells. The present study aimed to isolate bacteriophages against Klebsiella spp. and evaluate their efficacy against antibiotic-resistant species. Seventy-two antibiotic-resistant Klebsiella spp. were isolated from samples of patients who referred to the Ghaem Hospital (Mashhad, Iran). Lytic bacteriophages against Klebsiella spp. were isolated from wastewater of the septic tank of the same hospital. Bactericidal activity of phages against resistant Klebsiella spp. was tested in both liquid (tube method; after 1 and 24 h of incubation) and solid (double-layer agar plate method; after 24 h of incubation) phases. In each method, three different concentrations of bacteriophages (low: <104 PFU/mL, medium: 104-10 7 PFU/mL, and high: >107 PFU/mL) were used. Bacteriophages showed promising bactericidal activity at all assessed concentrations, regardless of the test method and duration of incubation. Overall, bactericidal effects were augmented at higher concentrations. In the tube method, higher activity was observed after 24 h of incubation compared to the 1-h incubation. The bactericidal effects were also higher in the tube method compared to the double-layer agar plate method after 24 h of incubation. The findings of the present study suggest that bacteriophages possess effective bactericidal activity against resistant Klebsiella spp. These bactericidal activities are influenced by phage concentration, duration of incubation, and test method. ©2011 with author. Published by TheScientificWorld.

Item Type: Article
Additional Information: Cited By :10 Export Date: 16 February 2020 Correspondence Address: Sabouri Ghannad, M.; Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; email:
Uncontrolled Keywords: Antibiotic resistance Bacteriophage Klebsiella agar probiotic agent article bactericidal activity bacterium isolation concentration (parameters) drug efficacy incubation temperature liquid culture nonhuman prevalence septic tank virus isolation waste water Anti-Bacterial Agents Bacteriophages Drug Resistance, Bacterial Microbial Sensitivity Tests Virus Cultivation Bacteria (microorganisms) Eukaryota
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 18 Apr 2020 09:31
Last Modified: 18 Apr 2020 09:31

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