The potential inhibitory effect of cuminum cyminum, ziziphora clinopodioides and nigella sativa essential oils on the growth of aspergillus fumigatus and aspergillus flavus

Khosravi, A. R. and Minooeianhaghighi, M. H. and Shokri, H. and Emami, S. A. and Alavi, S. M. and Asili, J. (2011) The potential inhibitory effect of cuminum cyminum, ziziphora clinopodioides and nigella sativa essential oils on the growth of aspergillus fumigatus and aspergillus flavus. Brazilian Journal of Microbiology, 42 (1). pp. 216-224.

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Abstract

The goals of this study were to evaluate the effectiveness of Cuminum cyminum, Ziziphora clinopodioides and Nigella sativa essential oils to inhibit the growth of Aspergillus fumigatus and A. flavus and to evoke ultrastructural changes. The fungi were cultured into RPMI 1640 media in the presence of oils at concentrations of 8, 6, 5, 4, 3, 2, 1.5, 1.25, 1, 0.75 and 0.5 mg/ml in broth microdilution and 2, 1.5, 1 and 0.5 mg/ml in broth macrodilution methods with shaking for 48 h at 28°C. Conidial and mycelial samples exposed to 0.25, 0.5, 1, 1.5 and 2 mg essential oils/ml for 5 days in 2 yeast extract granulated plus 15 Saccharose media were processed for transmission electron microscopy (TEM). Based on broth dilution methods, C. cyminum and to a lesser extent Z. clinopodioides oils exhibited the strongest activity against A. fumigatus and A. flavus with MIC90 ranging from 0.25 to 1.5 mg/ml, while the oil from N. sativa exhibited relatively moderate activity against two above fungi with MIC90 ranging from 1.5 to 2 mg/ml. The main changes observed by TEM were in the cell wall, plasma membrane and membranous organelles; in particular, in the nuclei and mitochondria. These modifications in fungal structure were associated with the interference of the essential oils with the enzymes responsible for cell wall synthesis, which disturbed normal growth. Moreover, the essential oils caused high vacuolation of the cytoplasm, detachment of fibrillar layer of cell wall, plasma membrane disruption and disorganization of the nuclear and mitochondrial structures. Aspergillus fumigatus and A. flavus growth inhibition induced by these oils were found to be well-correlated with subsequent morphological changes of the fungi exposed to different fungistatic concentrations of the oils. Our results show the anti-Aspergillus activities of C. cyminum, Z. clinopodioides and N. sativa essential oils, which strengthens the potential use of these substances as anti-mould in the future.

Item Type: Article
Additional Information: Cited By :33 Export Date: 16 February 2020 Correspondence Address: Khosravi, A. R.; Mycology Research Center, Faculty of Veterinary Medicine, Azadi street, Tehran, Iran; email: khosravi@ut.ac.ir
Uncontrolled Keywords: Antifungal activity Aspergillus Cuminum cyminum Essential oil Nigella sativa Ziziphora clinopodioides Aspergillus flavus Aspergillus fumigatus Fungi Ziziphora
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 04 May 2020 08:18
Last Modified: 04 May 2020 08:18
URI: http://eprints.mums.ac.ir/id/eprint/17038

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