On the track of mesothelial progenitor cells from the peritoneal cavity transplanted to immunodefficient mice

Moallem, S. A. and Jahangiri, L. (2007) On the track of mesothelial progenitor cells from the peritoneal cavity transplanted to immunodefficient mice. Pakistan Journal of Biological Sciences, 10 (17). pp. 2790-2799.

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Abstract

Mesothelial progenitor cells have been reported to reside in either the monolayer of mesothelium, submesothelium or within the peritoneal cavity as free floating cells. As a putative plasticity has been reported for the mesothelial progenitor cells and considering the potential implications of the establishment of a novel resource of stem /progenitor cells in gene and cell therapeutics and tissue engineering, we conducted an in vivo tracking of transplanted mesothelial cells. In order to induce immunodeficiency, the recipient mice were treated with 32 mg kg-1 of daily Cyclosporine. On days 14, 30 and 60 post transplantation, brain, heart, skeletal muscle and lung tissues were screened by a modified FISH method directed to the Y chromosome of donor cells. Fluorescence harboring cells were analyzed by flow cytometry and fluorescent microscopy. The data confirmed by PCR, demonstrated the existence morphology alteration of the donor cells in various organs of the recipient mice, notably in the skeletal muscle and lung and less in the heart and brain. Immunostaining of recovered cells from the nervous recipient tissues suggests differentiation of mesothelial cells in the new microenvironment. © 2007 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Moallem, S.A.; School of Pharmacy, P.O. Box 91775-1365, Mashhad, Iran
Uncontrolled Keywords: Mesothelial cells Mice Peritoneum Progenitor cell transplantation Stem cells Mus
Subjects: WI Digestive System
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 02 May 2020 03:25
Last Modified: 02 May 2020 03:25
URI: http://eprints.mums.ac.ir/id/eprint/17057

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