Rapid and low-cost colorimetric method using 2,3,5-triphenyltetrazolium chloride for detection of multidrug-resistant Mycobacterium tuberculosis

Mohammadzadeh, A. and Farnia, P. and Ghazvini, K. and Behdani, M. and Rashed, T. and Ghanaat, J. (2006) Rapid and low-cost colorimetric method using 2,3,5-triphenyltetrazolium chloride for detection of multidrug-resistant Mycobacterium tuberculosis. Journal of Medical Microbiology, 55 (12). pp. 1657-1659.

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Abstract

A rapid and inexpensive method for the detection of drug resistance in Mycobacterium tuberculosis is essential for the effective control of tuberculosis. The aim of this study was to evaluate a colorimetric method using 2,3,5-triphenyltetrazolium chloride (TTC) for antibiotic susceptibility testing of M. tuberculosis isolates. Eleven multidrug-resistant (MDR) isolates of M. tuberculosis and 12 isolates which were susceptible to rifampicin (RIF) and isoniazid (INH) were used. The test was performed with a critical concentration of 0.2 μg ml-1 for INH and 2.0 μg ml-1 for RIF in 7H9GC broth with 0.625 μg TTC ml-1. Each isolate was inoculated under these conditions and inspected daily for colour changes; the results were obtained after a mean of 4.9 days. The sensitivity and specificity of this method were 100 and 92, respectively, for both antibiotics. Considering the speed, technical ease and cost-effectiveness of this method, the TTC assay is a good alternative method for drug susceptibility testing of M. tuberculosis isolates. © 2006 SGM.

Item Type: Article
Additional Information: Cited By :21 Export Date: 16 February 2020 CODEN: JMMIA Correspondence Address: Behdani, M.; Education Department, Pasteur Institute of Iran, Tehran, Iran; email: Behdani73042@yahoo.com
Uncontrolled Keywords: isoniazid rifampicin triphenyltetrazolium antibiotic sensitivity article bacterium detection colorimetry cost effectiveness analysis inoculation multidrug resistance Mycobacterium tuberculosis nonhuman priority journal sensitivity and specificity Antibiotics, Antitubercular Bacteriological Techniques Drug Resistance, Multiple, Bacterial Humans Microbial Sensitivity Tests Rifampin Tetrazolium Salts Tuberculosis
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 03 May 2020 03:40
Last Modified: 03 May 2020 03:40
URI: http://eprints.mums.ac.ir/id/eprint/17062

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