Chrysin reduces proliferation and induces apoptosis in the human prostate cancer cell line pc-3

Samarghandian, S. and Afshari, J. T. and Davoodi, S. (2011) Chrysin reduces proliferation and induces apoptosis in the human prostate cancer cell line pc-3. Clinics, 66 (6). pp. 1073-1079.

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Abstract

INTRODUCTION: Honey is a common household product with many medicinal uses described in traditional medicine. Only recently has its antioxidant properties and preventive effects against disease been highlighted. Chrysin is a natural flavone commonly found in honey that has been shown to be an antioxidant agent. In this study, we investigated the antiproliferative and apoptotic effects of honey and chrysin on cultured human prostate cancer cells. METHODS: Cells were cultured in RPMI medium and treated with different concentrations of honey and chrysin for three consecutive days. Cell viability was quantitated by the 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The percentage of apoptotic cells was determined by flow cytometry using Annexin Vfluorescein isothiocyanate. RESULTS: The MTT assay revealed that both compounds had an antiproliferative effect on PC-3 cells in a dose- and time-dependent manner. The IC 50 values for honey and chrysin against PC-3 cells were 2.5 and 24.5 after 48 h and 1.8 and 8.5 after 72 h, respectively. Chrysin induced apoptosis in PC-3 cells, as determined by flow cytometry. CONCLUSION: Our results suggest that honey has anti-proliferative effects on prostate cancer cells and the effects are mainly due to chrysin. Therefore, chrysin may be a potential compound for both cancer prevention and treatment. Further in vivo investigation is needed to support the use of chrysin in cancer therapy. © 2011 CLINICS.

Item Type: Article
Additional Information: Cited By :115 Export Date: 16 February 2020 Correspondence Address: Samarghandian, S.; Department of Physiology, School of Medicine, Mashhad University Medical Sciences, Mashhad, Iran; email: samarghandians@mums.ac.ir
Uncontrolled Keywords: Apoptosis Chrysin Cytotoxicity Honey PC-3 antioxidant flavonoid lipocortin 5 analysis of variance apitherapy article carcinoma cell proliferation cell survival drug effect flow cytometry human male methodology prostate tumor time tumor cell line Annexin A5 Antioxidants Cell Line, Tumor Flavonoids Humans Prostatic Neoplasms Time Factors
Subjects: WJ Urogenital System
QX Parasitology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 20 Apr 2020 09:25
Last Modified: 20 Apr 2020 09:25
URI: http://eprints.mums.ac.ir/id/eprint/17114

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