Galbanic acid from Ferula szowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus

Shahverdi, A. R. and Fakhimi, A. and Zarrini, G. and Dehghan, G. and Iranshahi, M. (2007) Galbanic acid from Ferula szowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus. Biological and Pharmaceutical Bulletin, 30 (9). pp. 1805-1807.

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Abstract

In this study the enhancement effect of Ferula szowitsiana roots' acetone extract on the antibacterial activity of penicillin G and cephalexin was evaluated against Staphylococcus aureus. Disk diffusion and broth dilution methods were used to determine the antibacterial activity of these antibiotics in the absence and presence of plant extract and its various fractions separated by TLC plate. The active component of plant extract involved in enhancement of penicillin G's and cephalexin's activities had Rf=0.336 on a TLC plate. The spectral data (1H-, 13C-NMR) of this compound revealed that this compound was 7-6-(β-carboxyethyl)-5-isopropylidene-1,2- dimethylcyclo-hexylmethoxycoumarin (galbanic acid), previously isolated from Ferula assa-foetida. In the presence of sub-inhibitory concentration of galbanic acid (100 μg/ml) the MIC of penicillin G for S. aureus decreased from 64 to 1 (a sixteen four-fold decrease) and for cephalexin from 128 to 1 μg/ml (a one hundred twenty eight-fold decrease). The highest fold decrease in MIC was observed for cephalexin in combination of galbanic acid against test strain. These results signify that the low concentration of galbanic acid (100 μg/ml) potentiates the antimicrobial action of penicillin G and cephalexin suggesting a possible utilization of these compounds in combination therapy against S. aureus. © 2007 Pharmaceutical Society of Japan.

Item Type: Article
Additional Information: Cited By :43 Export Date: 16 February 2020 CODEN: BPBLE Correspondence Address: Shahverdi, A.R.; Department of Pharmaceutical Biotechnology, Medicinal Plant Research Center, Medical Sciences/University of Tehran, Tehran 1417614411, Iran; email: shahverd@sina.tums.ac.ir
Uncontrolled Keywords: Antibacterial activity Cephalexin Ferula szowitsiana Penicillin G Staphylococcus aureus Synergism 7 6 (beta carboxyethyl) 5 isopropylidene 1,2 dimethylcyclohexylmethoxycoumarin acetone antibiotic agent cefalexin Ferula szowitsiana extract galbanic acid plant extract unclassified drug article bacterial strain broth dilution method carbon nuclear magnetic resonance controlled study disk diffusion method drug isolation drug potentiation medicinal plant minimum inhibitory concentration proton nuclear magnetic resonance spectroscopy technique thin layer chromatography Anti-Bacterial Agents Chromatography, Thin Layer Coumarins Ferula Iran Microbial Sensitivity Tests Plant Extracts Plant Roots Solvents
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib7 lib7
Date Deposited: 29 Apr 2020 03:51
Last Modified: 29 Apr 2020 03:51
URI: http://eprints.mums.ac.ir/id/eprint/17120

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