Study of serum bactericidal and splenic activity of Total-OMP- CagA combination from Brucella abortus and Helicobacter pylori in BALB/c mouse model

Abadi, A. H. and Mahdavi, M. and Khaledi, A. and Esmaeili, S. A. and Esmaeili, D. and Sahebkar, A. (2018) Study of serum bactericidal and splenic activity of Total-OMP- CagA combination from Brucella abortus and Helicobacter pylori in BALB/c mouse model. Microbial Pathogenesis, 121. pp. 100-105.

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Abstract

Background: Brucella is a Gram-negative and facultative intracellular organism that causes brucellosis, a common zoonotic disease. Over 500,000 people are annually affected by brucellosis. Brucella is highly infectious through inhalation route; for this reason it is used for biological warfare aims. This study aimed to study the serum bactericidal and splenic activity of Total-OMP-r CagA immunogens from Brucella abortus and Helicobacter pylori in a BALB/c mouse model. Methods: Immunization of BALB/c mice was performed with immunogenic proteins three times subcutaneously (S.C.) at 14-day intervals. The protective effects of two component vaccines with CpG adjuvant were evaluated after mice were challenged with H. pylori ss1 and Brucella abortus strain 544. The specific IgG1 and IgG2a antibodies in sera were assessed using ELISA test. For measuring the antigen-specific IL-4, IL-12 and IFN-γ responses in sera of immunized mice after challenge, RT-PCR technique was applied. Twenty days after the challenge, mice were killed then gastric, splenic and serum samples were assessed and bacterial colony count was measured based on the pour plate count agar. Results: The results indicated that rCagA + OMP decreased bacterial colonization in these tissues, and significant difference was observed between test and control groups (p value˂0.001). Conclusion: Our results showed that the combination vaccine was effective against an oral exposure and the bacterial burden in the spleen, serum and gastric tissues were reduced in mice immunized with the Total- OMP-CagA. © 2018

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 CODEN: MIPAE Correspondence Address: Esmaeili, D.; Department of Microbiology and Applied Microbiology Research Center, Systems biology and poisonings institute, Baqiyatallah University of Medical Sciences, Nosrati Alley, Sheikh Bahai South Avenue, Mollasadra St, Vanak Sq, Iran; email: esm114@gmail.com
Uncontrolled Keywords: Brucella abortus Helicobacter pylori Total-OMP- CagA CagA protein gamma interferon immunoglobulin G1 antibody immunoglobulin G2a antibody immunological adjuvant interleukin 12 interleukin 4 outer membrane protein bacterial antigen bacterial protein bacterial vaccine bacterium antibody Brucella vaccine cagA protein, Helicobacter pylori cytokine immunoglobulin G animal experiment animal model animal tissue antigen specificity Article bacterial count brucellosis combination drug therapy controlled study drug efficacy enzyme linked immunosorbent assay female Helicobacter infection immunization infection prevention mouse mouse model nonhuman priority journal reverse transcription polymerase chain reaction serum bactericidal activity animal Bagg albino mouse blood disease model immunology spleen Animals Antibodies, Bacterial Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Bacterial Vaccines Blood Bactericidal Activity Colony Count, Microbial Cytokines Disease Models, Animal Helicobacter Infections Interferon-gamma Mice Mice, Inbred BALB C
Subjects: WC Communicable Diseases
WH Hemic and Lymphatic System
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 10 Jun 2020 07:17
Last Modified: 10 Jun 2020 07:17
URI: http://eprints.mums.ac.ir/id/eprint/17154

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