Contribution of KCTD12 to esophageal squamous cell carcinoma

Abbaszadegan, M. R. and Taghehchian, N. and Li, L. and Aarabi, A. and Moghbeli, M. (2018) Contribution of KCTD12 to esophageal squamous cell carcinoma. BMC Cancer, 18 (1).

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Background: It has been shown that the expression of potassium channel tetramerization domain containing 12 (KCTD12) as a regulator of GABAB receptor signaling is reversely associated with gastrointestinal stromal tumors. In present study we examined the probable role of KCTD12 in regulation of several signaling pathways and chromatin remodelers in esophageal squamous cell carcinoma (ESCC). Methods: KCTD12 ectopic expression was done in KYSE30 cell line. Comparative quantitative real time PCR was used to assess the expression of stem cell factors and several factors belonging to the WNT/NOTCH and chromatin remodeling in transfected cells in comparison with non-transfected cells. Results: We observed that the KCTD12 significantly down regulated expression of NANOG, SOX2, SALL4, KLF4, MAML1, PYGO2, BMI1, BRG1, MSI1, MEIS1, EGFR, DIDO1, ABCC4, ABCG2, and CRIPTO1 in transfected cells in comparison with non-transfected cells. Migration assay showed a significant decrease in cell movement in ectopic expressed cells in comparison with non-transfected cells (p = 0.02). Moreover, KCTD12 significantly decreased the 5FU resistance in transfected cells (p = 0.01). Conclusions: KCTD12 may exert its inhibitory role in ESCC through the suppression of WNT /NOTCH, stem cell factors, and chromatin remodelers and can be introduced as an efficient therapeutic marker. © 2018 The Author(s).

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 CODEN: BCMAC Correspondence Address: Moghbeli, M.; Mashhad University of Medical Sciences, Department of Modern Sciences and Technologies, Faculty of MedicineIran; email:
Uncontrolled Keywords: Chromatin remodeling Esophageal cancer NOTCH Self-renewal Stem cell WNT BMI1 protein breast cancer resistance protein BRG1 protein cell protein CRIPTO1 protein DIDO1 protein epidermal growth factor receptor homeobox protein Meis1 KCTD12 protein kruppel like factor 4 MAML1 protein MSI1 protein multidrug resistance associated protein 4 Notch receptor potassium channel PYGO2 protein SALL4 protein stem cell factor transcription factor NANOG transcription factor Sox2 unclassified drug Wnt protein fluorouracil homeodomain protein KCTD12 protein, human protein tumor marker Article cell migration assay cell motion chromatin assembly and disassembly controlled study disease association down regulation ectopic expression esophageal squamous cell carcinoma genetic transfection KYSE-30 cell line protein expression protein function quantitative analysis real time polymerase chain reaction signal transduction cancer stem cell cell proliferation drug resistance gene expression regulation genetics human metabolism tumor cell line Biomarkers, Tumor Cell Line, Tumor Cell Movement Drug Resistance, Neoplasm Ectopic Gene Expression Gene Expression Regulation, Neoplastic Homeodomain Proteins Humans Neoplastic Stem Cells Proteins Receptors, Notch Wnt Proteins
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 10 Jun 2020 06:47
Last Modified: 10 Jun 2020 06:47

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