Improved anticancer efficacy of epirubicin by magnetic mesoporous silica nanoparticles: in vitro and in vivo studies

Ansari, L. and Jaafari, M. R. and Bastami, T. R. and Malaekeh-Nikouei, B. (2018) Improved anticancer efficacy of epirubicin by magnetic mesoporous silica nanoparticles: in vitro and in vivo studies. Artificial Cells, Nanomedicine and Biotechnology, 46 (sup2). pp. 594-606.

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Abstract

The development of magnetic nanoparticles as delivery carriers to magnetically accumulate anticancer drug in cancer tissue has attracted immense interest. In the present study, magnetic mesoporous silica nanoparticles (MMSNs) with magnetite core and silica shell were synthesized. The obtained MMSNs were characterized by DLS, XRD, FT-IR, TEM and VSM in order to investigate the nanoparticle characteristics. With the focus on in vivo validation of such magnetic drug delivery systems, we selected epirubicin (EPI) as the drug. The anticancer properties of EPI-loaded MMSNs were evaluated in a C-26 colon carcinoma model. Alongside monitoring of drug in the tissues with animal imaging system, the tissue distribution was also determined quantitavely. The average size of MMSNs determined with TEM images was about 18.68 ± 2.31 nm. The cellular uptake test indicated that geometric mean fluorescence intensity (MFI) of cells treated with MMSN + EPI in presence of external magnetic field was increasing 27 compared with free EPI. In addition, treatment with drug-loaded MMSNs with the aid of external magnetic gradient had significantly higher inhibition efficacy towards tumor growth than the free EPI treated mice. The targeted drug delivery through external magnet-attraction using EPI-loaded MMSNs resulted in high tumor cell uptake, which leads to elimination of cancer cells effectively. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :3 Export Date: 16 February 2020 Correspondence Address: Malaekeh-Nikouei, B.; School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box 91775-1365, Iran; email: malaekehb@mums.ac.ir
Uncontrolled Keywords: biodistribution cancer therapy magnetic targeting Mesoporous silica nanoparticles superparamagnetic iron oxide nanoparticle Controlled drug delivery Diseases Iron oxides Magnetite Magnetite nanoparticles Mesoporous materials Nanomagnetics Silica nanoparticles Synthesis (chemical) Tumors Biodistributions Superparamagnetic iron oxide nanoparticles Targeted drug delivery antineoplastic agent epirubicin magnetic nanoparticle mesoporous silica nanoparticle drug carrier magnetite nanoparticle silicon dioxide animal cell animal experiment antineoplastic activity Article blood cell count body weight loss cancer cell cancer chemotherapy cancer inhibition cancer tissue cytotoxicity cytotoxicity assay drug delivery system drug efficacy drug release endocytosis erythrocyte flow cytometry Fourier transform infrared spectroscopy heart hemolysis assay human human cell IC50 imaging and display in vitro study in vivo study kidney liver lung magnetic field MC-26 cell line mean fluorescence intensity mouse MTT assay nonhuman particle size photon correlation spectroscopy reticuloendothelial system spleen synthesis therapy effect tissue distribution transmission electron microscopy tumor cell tumor growth X ray powder diffraction animal chemistry conformation female molecular model porosity tumor cell line Animals Antineoplastic Agents Cell Line, Tumor Drug Carriers Mice Models, Molecular Molecular Conformation
Subjects: QU Biochemistry
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 08 Jun 2020 05:41
Last Modified: 08 Jun 2020 05:41
URI: http://eprints.mums.ac.ir/id/eprint/17192

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