Effects of thymoquinone on sperm parameters, apoptosis, testosterone level, and oxidative stress in a mouse model of d-galactose-induced aging

Attari, S. S. and Mohammadi, S. and Ebrahimzadeh, A. and Hosseinzadeh, H. and Soukhtanloo, M. and Rajabzadeh, A. (2018) Effects of thymoquinone on sperm parameters, apoptosis, testosterone level, and oxidative stress in a mouse model of d-galactose-induced aging. Pharmaceutical Sciences, 24 (3). pp. 180-186.

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Effects of thymoquinone on sperm parameters, apoptosis, testosterone level, and oxidative stress in a mouse model of d-galactose-induced aging.pdf

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Abstract

Background: The aging process is accompanied by low secretion of sex hormones and testicular apoptosis. The antioxidant properties of thymoquinone (TQ) may prevent the effects of aging. Therefore, in the present study, the effects of different doses of TQ were investigated on sperm parameters, testosterone level, apoptosis, and oxidative stress in a mouse model of D-galactose-induced aging. Methods: In this experimental study, 30 adult male mice were randomly divided into 5 groups. The control group did not receive any injections, while the D-galactose group received an intraperitoneal injection of 300 mg/kg of D-galactose for 42 days. The TQ1-TQ3 groups received intraperitoneal injections of 5, 2.5, and 1.25 mg/kg of TQ plus Dgalactose, respectively for 14 days (from the 1st to the 14th day of the experiment). The morphometric analysis, testicular apoptosis examination, and sperm analysis were performed, and testosterone level, total antioxidant capacity, and malondialdehyde level were evaluated on day 42 of the experiment. Data were analyzed using SPSS. Results: Administration of TQ in the TQ1 group caused a significant difference in sperm parameters, compared to the D-galactose group (P<0.05). The lowest amount of positive tunnel cells was related to 5 mg/kg of TQ and the highest to 2.5 mg/kg of TQ. There was no significant difference in the parameters of seminal vesicles, epididymis, prostate, and testis between the groups (P>0.05). The malondialdehyde level were decreased in the TQ1-TQ3 groups, compared to the D-galactose group (P<0.001). On the other hand, the total antioxidant capacity was increased significantly in the TQ1 group, compared to the Dgalactose group (P<0.001). Conclusion: Administration of 5 mg of TQ for 14 days improved sperm quality and biochemical parameters, while reducing apoptotic cells of the testes in a mouse model of aging. © 2018 The Authors.

Item Type: Article
Additional Information: Cited By :5 Export Date: 16 February 2020 Correspondence Address: Mohammadi, S.; Neurogenic Inflammation Research Center, Mashhad University of Medical SciencesIran; email: mohammadish@mums.ac.ir
Uncontrolled Keywords: Aging Mice Spermatozoa Testis Thymoquinone galactose malonaldehyde testosterone adult animal experiment animal tissue apoptosis Article controlled study dose response drug effect epididymis male mouse nonhuman oxidative stress semen analysis sperm sperm quality spermatozoon count spermatozoon motility testis size testosterone blood level
Subjects: WJ Urogenital System
WT Geriatrics . Chronic Diseases
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 08 Jun 2020 04:51
Last Modified: 08 Jun 2020 04:51
URI: http://eprints.mums.ac.ir/id/eprint/17214

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