Absence of FLT3 mutations in Iranian adult T-cell leukemia/lymphoma patients

Ayatollahi, H. and Sadeghian, M. and Kooshyar, M. and Shirdel, A. and Rahimi, H. and Jafarian, A. and Ghazaei, S. and Soltani, N. and Shams, F. and Rad, N. M. and Shakeri, S. (2018) Absence of FLT3 mutations in Iranian adult T-cell leukemia/lymphoma patients. Medical Journal of the Islamic Republic of Iran, 32 (1). pp. 268-272.

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Abstract

Background: Adult T cell leukemia lymphoma (ATLL) is a rare disease, significantly linked to the infection by the human T-cell lymphotropic virus 1(HTLV-1). ATLL is typically preceded by decades of clinical latency during which infected cells accumulate selectable traits leading to a malignant transformation. Amongst all the HTLV-1 infected carriers only about 3-5 will develop ATLL. Despite the intensive attempt to improve the overall survival, ATLL remains one of worse prognosis among the hematologic malignancies. FMS like tyrosine kinase 3 internal tandem duplication (FLT3-ITD) mutations are mutations which are frequent among leukemic patients. We aimed to investigate the frequency of FLT3 mutation status in patients with acute type of ATLL which has not been studied yet. Methods: In this case control study 38 patients with acute type of ATLL were retrospectively analyzed between February 2015 and February 2017. Forty HTLV-1 positive patients were also used as control cases. Genomic DNA was extracted according to phenolchloroform protocol and two restriction fragment length polymorphism (RFLP) PCR reactions were set up to detect FLT3/ITD and FLT3/TKD mutations. Differences between variables were evaluated by the chi-square test and t test for categorical and continuous variables, respectively. SPSS software v. 15 was used for statistical analysis. All P values were two sided and values less than 0.05 were considered to be significant. Results: No FLT3 mutations were detected in acute type of ATLL patients. So far, not many studies have shown the frequency of FLT3 mutation in ATLL patients Conclusion: Therefore, we conclude that although FLT3 mutations are rather unusual in the acute type of ATLL patients, but other alternative mechanisms associated with ATLL remain to be further investigated. This study was a novel project regarding the analysis of FLT3 mutation in the field of ATLL research. © Iran University of Medical Sciences.

Item Type: Article
Additional Information: Export Date: 16 February 2020 CODEN: MJIIE Correspondence Address: Shakeri, S.; Cancer Molecular Pathology Research Center, Ghaem Medical Center Mashhad University of Medical SciencesIran; email: ssh.shakeri@gmail.com
Uncontrolled Keywords: Adult T cell leukemia Human T-cell lymphotropic virus 1 Iran
Subjects: WH Hemic and Lymphatic System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 08 Jun 2020 05:01
Last Modified: 08 Jun 2020 05:01
URI: http://eprints.mums.ac.ir/id/eprint/17216

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