The effects of tramadol administration on hippocampal cell apoptosis, learning and memory in adult rats and neuroprotective effects of crocin

Baghishani, F. and Mohammadipour, A. and Hosseinzadeh, H. and Hosseini, M. and Ebrahimzadeh-bideskan, A. (2018) The effects of tramadol administration on hippocampal cell apoptosis, learning and memory in adult rats and neuroprotective effects of crocin. Metabolic Brain Disease, 33 (3). pp. 907-916.

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Abstract

Tramadol, a frequently used pain reliever drug, present neurotoxic effects associated to cognitive dysfunction. Moreover, crocin has been reported to have neuroprotective effects. The aim of this study was to assess crocin’s capacity to protect learning, and memory abilities on tramadol-treated rats. A total of 35 rats were divided into five groups: Control, Saline, tramadol (50 mg/kg), tramadol + crocin(30 mg/kg), crocin groups and treated orally for 28 consecutive days. Morris water maze (MWM) and passive avoidance (PA) tests were done, followed by dissection of the rat’s brains for toluidine blue and TUNEL staining. In MWM test, tramadol group spent lower time and traveled shorter distance in the target quadrant (Q1) (P < 0.05). On the other side, the traveled distance in tramadol-crocin group was higher than tramadol (P < 0.05). In PA test, both the delay for entering the dark, and the total time spent in the light compartment decreased in tramadol comparing to the control group (P < 0.05), while it increased in tramadol-crocin compared with the tramadol group (P < 0.05). In tramadol-treated animals, the dark neurons (DNs) and apoptotic cells in CA1, CA3 and DG increased (P < 0.05), while concurrent intake of crocin decreased the number of DNs and apoptotic cells in these areas (P < 0.05). Crocin was able to improve learning and memory of tramadol-treated rats and also decreased DNs and apoptotic cells in the hippocampus. Considering these results, the potential capacity of crocin for decreasing side effects of tramadol on the nervous system is suggested. © 2018, The Author(s).

Item Type: Article
Additional Information: Cited By :10 Export Date: 16 February 2020 CODEN: MBDIE Correspondence Address: Ebrahimzadeh-bideskan, A.; Microanatomy Research Center, School of Medicine, Mashhad University of Medical SciencesIran; email: Ebrahimzadehba@mums.ac.ir
Uncontrolled Keywords: Apoptosis Crocin Memory Morris water maze Passive avoidance Tramadol tolonium chloride antioxidant carotenoid neuroprotective agent plant extract adult animal experiment animal model animal tissue Article brain nerve cell controlled study drug effect hippocampal CA1 region hippocampal CA2 region hippocampal CA3 region learning learning disorder light-dark test male memory disorder Morris water maze test nerve cell necrosis neuroprotection neurotoxicity nonhuman passive avoidance test rat treatment outcome TUNEL assay animal hippocampus maze test nerve cell Wistar rat Animals Antioxidants Carotenoids Maze Learning Memory Disorders Neurons Neuroprotective Agents Plant Extracts Rats, Wistar
Subjects: WL Nervous system
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 06 Jun 2020 07:22
Last Modified: 06 Jun 2020 07:22
URI: http://eprints.mums.ac.ir/id/eprint/17225

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