Transient expression of CCL21as recombinant protein in tomato

Beihaghi, M. and Marashi, H. and Bagheri, A. and Sankian, M. (2018) Transient expression of CCL21as recombinant protein in tomato. Biotechnology Reports, 17. pp. 10-15.

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Abstract

The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agro-infiltrated into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was confirmed by quantitative Real-time PCR. A recombinant CCL21 protein was immunogenically detected by western blot, dot blot and ELISA assay. And results showed that the foreign gene was expressed in the transformed leaves in high level. Also scratch assay was used to investigate the role of this protein in anti-metastatic function. The results demonstrated anti-metastatic of cancer cells in the presence of this protein. © 2017 The Authors

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Marashi, H.; College of Agriculture, Ferdowsi University of MashhadIran; email: marashi@um.ac.ir
Uncontrolled Keywords: Agroinfiltration Migration Recombinant vaccine Scratch assay Tomato Transient gene expression recombinant protein secondary lymphoid tissue chemokine Article cell infiltration cell migration cell proliferation cell survival controlled study dot hybridization enzyme linked immunosorbent assay gene construct genetic transfection immunogenicity incubation time nonhuman priority journal real time polymerase chain reaction RNA extraction sequence analysis transient expression Western blotting
Subjects: QV pharmacology
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 02 Jun 2020 08:07
Last Modified: 02 Jun 2020 08:07
URI: http://eprints.mums.ac.ir/id/eprint/17236

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