Association of genetic polymorphisms of PON1 and CETP with the presence of metabolic syndrome; the effects of genotypes on their serum activity and concentrations

Dizaji, B. F. and Rivandi, M. and Javandoost, A. and Saberi Karimian, M. and Raei, A. and Sahebkar, A. and Ferns, G. and Mobarhan, M. G. and Pasdar, A. (2018) Association of genetic polymorphisms of PON1 and CETP with the presence of metabolic syndrome; the effects of genotypes on their serum activity and concentrations. Egyptian Journal of Medical Human Genetics, 19 (1). pp. 43-48.

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Association of genetic polymorphisms of PON1 and CETP with the presence of metabolic syndrome; the effects of genotypes on their serum activity and concentrations.pdf

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Abstract

Background The Metabolic syndrome (MetS) is associated with an increased risk of cardiovascular disease and type 2 diabetes. PON1 and CETP genes may be involved in the pathogenesis of lipid metabolism and thus MetS. Several single nucleotide polymorphisms of genes were demonstrated to affect their function. Curcumin (diferuloylmethane) is a yellow pigment of turmeric that has shown numerous pharmacological activity against obesity and related conditions through anti-oxidant/anti-inflammatory properties. Objective We aimed to assess the association of these polymorphisms with metabolic syndrome and to investigate if these genetic variants were associated with an altered activity of PON1 and the protein levels of CETP at base line and after Curcumin supplementation. Methods The genotypes of PON1 and CETP polymorphisms were determined in 81 patients with MetS and 100 healthy individuals using ARMS-PCR and PCR-RFLP techniques. Results Individuals with different genotypes of the PON1 rs662, rs854560 and rs705379 polymorphisms did not differ with paraoxonase activity and CETP serum protein concentrations, either at baseline, or after intervention. Individuals with different PON1 rs854560 genotypes differ significantly in serum arylesterase activities (p =.037). There were statistically significant differences in genotype frequencies between cases and controls for CETP rs5882 genotypes (p-value =.034) but not in genotype frequencies and haplotypes for PON1 studied polymorphisms (p-value <.05). The odds ratio for CETP rs5882 was statistically significant using a dominant model. OR (95 CI) = 0.48 (025–0.92), p-value =.029. Conclusions There were no associations between the PON1 polymorphisms, or haplotypes with MetS. There was an association between CETPrs5882 and metabolic syndrome. AA genotype of CETPrs5882 appeared to be protective against MetS in our studied population. There were no association between the PON1 and CETP polymorphisms with PON1enzymatic activities and CETP protein levels at base line and after curcumin supplementation. © 2018

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Mobarhan, M.G.; Molecular Medicine Group, Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical SciencesIran; email: GhayourM@mums.ac.ir
Uncontrolled Keywords: CETP gene Metabolic syndrome PON1 gene Single nucleotide polymorphism (SNP) aryldialkylphosphatase 1 cholesterol ester transfer protein curcumin adult Article controlled study gene frequency genetic association genetic variability genotype haplotype human major clinical study metabolic syndrome X polymerase chain reaction randomized controlled trial (topic) restriction fragment length polymorphism single nucleotide polymorphism supplementation
Subjects: WD Nutrition Disease and metabolic diseases
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 26 May 2020 03:10
Last Modified: 26 May 2020 03:10
URI: http://eprints.mums.ac.ir/id/eprint/17258

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