Association of single nucleotide polymorphisms in miR-499 and miR-196a with susceptibility to breast cancer

Doulah, A. and Salehzadeh, A. and Mojarrad, M. (2018) Association of single nucleotide polymorphisms in miR-499 and miR-196a with susceptibility to breast cancer. Tropical Journal of Pharmaceutical Research, 17 (2). pp. 319-323.

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Abstract

Purpose: To investigate the relationship between miR-499 rs3746444 and miR-196a rs11614913 polymorphisms, and susceptibility to breast cancer in an Iranian population. Methods: This case-control study was performed on a population of 200 subjects comprising 100 breast cancer patients (case/observation group) and 100 healthy individuals (control group). Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was used to genotype these polymorphisms. P-values and odd ratios were determined, and p-values < 0.05 and odd ratios > 1 were considered statistically significant. Results: There were no significant differences between observation and control groups with respect to rs11614913 T/C polymorphism. The rs11614913 T allele was not identified as a risk factor for susceptibility to breast cancer (OR = 0.86, 95 CI = 0.85 - 1.3, p = 0.46). However, there were significant differences between observation and control groups with respect to rs3746444 T/C polymorphism. It was observed that cytosine-cytosine (CC) (OR = 4.5, 95 CI = 1.3 - 15.4, p = 0.06), and cytosine-thymine (CT) (OR = 1.9, 95 CI = 1- 3.6, p = 0.04) genotypes had protective influence against susceptibility to breast cancer. Conclusion: These results indicate that CC and CT genotypes are associated with reduced risk of breast cancer. In particular, the presence of C allele is significantly associated with a low risk of breast cancer. These findings may provide useful information for prevention and early diagnosis of breast cancer. © 2018, Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Doulah, A.; Department of Nursing, Faculty of Nursing and Midwifery, Ahvaz Branch, Islamic Azad UniversityIran; email: hdoulah@yahoo.com
Uncontrolled Keywords: ARMS-PCR Breast cancer Early diagnosis miR-196a rs11614913 miR-499 rs3746444 Polymorphism microRNA microRNA 196a microRNA 499 unclassified drug adult allele Article cancer susceptibility case control study controlled study DNA isolation female gene amplification gene frequency genetic association genetic risk genotype human major clinical study polymerase chain reaction single nucleotide polymorphism
Subjects: WP Gynecology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 26 May 2020 03:22
Last Modified: 26 May 2020 03:22
URI: http://eprints.mums.ac.ir/id/eprint/17261

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