High prevalence of candiduria due to non-albicans Candida species among diabetic patients: A matter of concern?

Esmailzadeh, A. and Zarrinfar, H. and Fata, A. and Sen, T. (2018) High prevalence of candiduria due to non-albicans Candida species among diabetic patients: A matter of concern? Journal of Clinical Laboratory Analysis, 32 (4).

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Abstract

Background: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. Method: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus. The specimens were centrifuged and the sediments were examined by direct examination and cultured on Sabouraud dextrose agar. The plates were incubated for 2-3 days at 35°C. The Candida colonies were counted and purified using CHROMagar Candida. The isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system. Results: Of the 400 urine specimens, 40 (10) had positive cultures for Candida species with a colony count of ≥1 × 103 colony forming units (CFU)/mL. The frequencies of the Candida species were as follows: C. albicans (n = 19, 47.5), C. glabrata (n = 15, 37.5), C. kefyer (n = 4, 10) and C. krusei (n = 2, 5). Seventy-three (88) of the patients with candiduria had hemoglobin A1c (HbA1c) levels above 7. Conclusion: The rate of candiduria was relatively high in type 2 diabetic patients and they were also suffering from a lack of proper blood glucose control. Although the frequency of non-albicans Candida species had not significantly higher than C. albicans, however, they obtained more from those with symptomatic candiduria. © 2017 Wiley Periodicals, Inc.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 CODEN: JCANE Correspondence Address: Zarrinfar, H.; Allergy research center, Mashhad University of Medical SciencesIran; email: Zarrinfarh@mums.ac.ir
Uncontrolled Keywords: candiduria diabetic patients MALDI-TOF Mashhad non-albicans Candida hemoglobin A1c Article Candida Candida albicans Candida glabrata Candida kefyer colony forming unit controlled study cross-sectional study diabetic patient female fungus culture fungus identification fungus isolation glucose blood level human major clinical study male matrix assisted laser desorption ionization time of flight mass spectrometry non insulin dependent diabetes mellitus Pichia kudriavzevii prevalence urinalysis candidiasis complication Iran matrix-assisted laser desorption-ionization mass spectrometry microbiology molecular typing urinary tract infection Cross-Sectional Studies Diabetes Mellitus, Type 2 Humans Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Urinary Tract Infections
Subjects: WK Endocrine System
QX Parasitology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 20 May 2020 09:00
Last Modified: 20 May 2020 09:00
URI: http://eprints.mums.ac.ir/id/eprint/17271

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