The effect of crocin on testicular tissue and sperm parameters of mice offspring from mothers exposed to atrazine during pregnancy and lactation periods: An experimental study

Fani, M. and Mohammadipour, A. and Ebrahimzadeh-Bideskan, A. (2018) The effect of crocin on testicular tissue and sperm parameters of mice offspring from mothers exposed to atrazine during pregnancy and lactation periods: An experimental study. International Journal of Reproductive BioMedicine, 16 (8). pp. 519-528.

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Abstract

Background: Atrazine as a herbicide may affect the human’s health. Crocin may protect atrazine-induced damages. Objective: The aim of this study was to evaluate the effects of atrazine on mice testicular tissue and sperm parameters and protective effects of Crocin on probably atrazine-induced damages. Materials and Methods: in this experimental study, 24 pregnant Balb/c mice were randomly divided to 4 groups: I: Atrazine (10 mg/kg), II: Atrazine-Crocin, III: Crocin (10mg/kg) and IV: Normal saline. Administrations were done daily by gavage during pregnancy and lactation. In the end, two male offspring were randomly selected from every mother and sacrificed respectively on 23 and 75 postnatal days. Then, their epididymides were removed for sperm parameters investigation and their testes were prepared to evaluate apoptosis by means of TUNEL technique. Results: The mean number of sperms in the atrazine group was lower compared to other groups and increased in the atrazine-crocin group compared with atrazine group significantly (p=0.001). Sperm abnormality was increased in the atrazine group compared with the normal saline group and decreased in the atrazine-crocin group compared with atrazine group significantly (p≤0.001). TUNEL-positive spermatogonia in 23 days old offspring increased significantly in the atrazine group compared with other groups (p=0.03). TUNEL-positive spermatogenic cells in 75 days old offspring was significantly increased in the atrazine group compared with the saline group (p≤0.001). Conclusion: Atrazine exposure may lead to decrease the number of sperms, increase sperms abnormality, spermatogenic cell apoptosis and height of germinal epithelium. These complications may improve by crocin administration. © 2018, Research and Clinical Center for Infertitlity. All rights reserved.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Ebrahimzadeh-Bideskan, A.; Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical SciencesIran; email: EbrahimzadehBA@mums.ac.ir
Uncontrolled Keywords: Apoptosis Atrazine Crocin Mouse Spermatogenesis antioxidant adult animal experiment animal model animal tissue antioxidant activity Article atrazine induced damage chemically induced disorder controlled study DNA damage epididymis female germ layer height lactation male microscopy nonhuman oxidative stress pregnancy semen abnormality semen analysis sperm quality spermatogonium spermatozoon count testis size testis tissue testis weight TUNEL assay
Subjects: WJ Urogenital System
WQ Obstetrics
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 20 May 2020 07:01
Last Modified: 20 May 2020 07:01
URI: http://eprints.mums.ac.ir/id/eprint/17274

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