Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in the p19 Function

Ghazizadeh, E. and Oskuee, R. K. and Jaafari, M. R. and Hosseinkhani, S. (2018) Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in the p19 Function. Scientific Reports, 8 (1).

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Abstract

The present study aim to design a liposomal electrochemical sensor using 1, 2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and dioleoylphosphatidylethanolamine(DOPE), chimeric probes and p19, it has been considered as a caliper molecule as well. Also the competitor structural hybrid (RNA) was used to detect three types of miRs in one screen printed electrode modified by gold nanoparticle (SCPE/GNP). In this purpose, the sensor signal stabilized when the cationic DOTAP-DOPE with hybrids of the chimeric probes (Stem, M-linear) sandwiched in order to detect 221-124a miRs. Given the lack of accessibility to RNA-miRs segments of chimeric probes, p19 inhibited the electrochemical reaction and shifted signal to off. After that p19 connected with the free hybrid of T-linear/21miR (just RNA) as competing for structure and the signal was shifted to ON, again. In this study, the electrochemical measurements were performed between the potentials at-0.4 V and +0.4 V with 1 mM Fe(CN)6-3-/4 which DOTAP-DOPE acted as an enhancer layer in the electrostatically reaction. This sensor determines as low as 0.4 fM of miRNA with high selectivity and specificity for sequential analysis of 124a-221-21 miRs in just 2 h. © 2018 The Author(s).

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Jaafari, M.R.; Biotechnology Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical SciencesIran; email: JafariMR@mums.ac.ir
Uncontrolled Keywords: 1,2-dielaidoylphosphatidylethanolamine dioleoyl phosphatidylethanolamine gold liposome metal nanoparticle microRNA phosphatidylethanolamine recombinant protein chemistry electrochemical analysis genetic procedures genetics human limit of detection metabolism procedures Biosensing Techniques Electrochemical Techniques Humans Liposomes Metal Nanoparticles MicroRNAs Phosphatidylethanolamines Recombinant Proteins
Subjects: QU Biochemistry
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 20 May 2020 04:26
Last Modified: 20 May 2020 04:26
URI: http://eprints.mums.ac.ir/id/eprint/17297

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