Long segment detection of HTLV-1 genome based on the fluorescence quenching technique

Ghobadi, M. Z. and Mozhgani, S. H. and Hakimian, F. and Norouzi, M. and Rezaee, S. A. and Ghourchian, H. (2018) Long segment detection of HTLV-1 genome based on the fluorescence quenching technique. Heliyon, 4 (12).

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Abstract

Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluorescence quenching of graphene oxide by gold nanoparticles. The fluorescence signal of unmodified graphene oxide was measured before and after hybridization of target and probes functionalized with gold nanoparticles. The limit of detection of the biosensor was determined to be around 10 pg/mL. The specific design for long segment of target assures the selectivity of biosensor. Our results proposed that further development may be useful to detect other viruses. © 2018

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Ghourchian, H.; Institute of Biochemistry and Biophysics, University of TehranIran; email: ghourchian@ut.ac.ir
Uncontrolled Keywords: Analytical chemistry Biotechnology Virology
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 20 May 2020 04:28
Last Modified: 20 May 2020 04:28
URI: http://eprints.mums.ac.ir/id/eprint/17298

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