Hypnotic effect of red cabbage (Brassica oleracea) on pentobarbital-induced sleep in mice

Hosseini, A. and Sobhanifar, M. A. and Forouzanfar, F. and Aghaee, A. and Rakhshandeh, H. (2018) Hypnotic effect of red cabbage (Brassica oleracea) on pentobarbital-induced sleep in mice. Journal of Pharmacy and Bioallied Sciences, 10 (1). pp. 48-53.

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Objective: The present study was performed to investigate the effect of hydroalcoholic extract of red cabbage and its fractions on sleeping behavior in mice. Materials and Methods: The extract and its fractions were injected to mice and sleep duration as well as sleep latency were recorded. Furthermore, toxicity of the extract was determined both in vivo and in vitro. Results: The extract increased sleep duration at doses of 50-200mg/kg (P 0.001). This observed hypnotic effect was comparable to that of diazepam (3mg/kg) (P 0.001 in comparison with control group). Ethyl acetate, n-butanol, and aqueous fractions could increase sleep duration (P 0.001). The sleep latency was decreased by the extract (P 0.001) and only ethyl acetate fraction (P 0.001). LD50 value for red cabbage extract was 2.4g/kg. There was no toxic effect on viability of cultured neuronal cells (PC12). Rotarod test results showed that there were no significant differences between the extract groups and the control group. Conclusion: The results suggest that red cabbage potentiates pentobarbital hypnosis without any toxic effect. The main component(s) responsible for this effect is most likely to be intermediate polar agent(s) such as flavonoids, which are found in ethyl acetate fraction of this plant. © 2018 Wolters Kluwer Medknow Publications. All rights reserved.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Rakhshandeh, H.; Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical SciencesIran; email: Rakhshandehh@mums.ac.ir
Uncontrolled Keywords: Diazepam PC12 pentobarbital red cabbage sleep acetic acid ethyl ester Brassica oleracea extract butanol flumazenil hypnotic sedative agent plant extract unclassified drug animal cell animal experiment Article Brassica oleracea controlled study drug effect hypnosis in vitro study in vivo study LD50 male mouse nerve cell culture nonhuman priority journal rotarod test sleep latency sleep time
Subjects: WL Nervous system
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 17 May 2020 08:54
Last Modified: 17 May 2020 08:54
URI: http://eprints.mums.ac.ir/id/eprint/17329

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