Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Kazemi, M. and Azami, M. and Johari, B. and Ahmadzadehzarajabad, M. and Nazari, B. and Kargozar, S. and Hajighasemlou, S. and Mozafari, M. and Soleimani, M. and Samadikuchaksaraei, A. and Farajollahi, M. (2018) Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs). International Journal of Applied Ceramic Technology, 15 (6). pp. 1427-1438.

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Abstract

In our previous study, a three-dimensional gelatin/bioactive glass nanocomposite scaffold with a total porosity of about 85 and pore sizes ranging from 200 to 500 μm was prepared through layer solvent casting combined with lamination technique. The aim of this study was to evaluate in vitro biocompatibility and in vivo bone regeneration potential of these scaffolds with and without endothelial cells when implanted into a critical-sized rat calvarial defect. MTT assay, SEM observation, and DAPI staining were used to evaluate cell viability and adhesion in macroporous scaffolds and results demonstrated that the scaffolds were biocompatible enough to support cell attachment and proliferation. To investigate the in vivo osteogenesis of the scaffold, blank scaffolds and endothelial/scaffold constructs were implanted in critical-sized defects, whereas in control group defects were left untreated. Bone regeneration and vascularization were evaluated at 1, 4, and 12 weeks postsurgery by histological, immunohistochemical, and histomorphometric analysis. It was shown that both groups facilitated bone growth into the defect area but improved bone regeneration was seen with the incorporation of endothelial cells. The data showed that the porous Gel/BaG nanocomposite scaffolds could well support new bone formation, indicating that the proposed strategy is a promising alternative for tissue-engineered bone defects. © 2018 The American Ceramic Society

Item Type: Article
Additional Information: Cited By :5 Export Date: 16 February 2020 Correspondence Address: Farajollahi, M.; Cellular and Molecular Research Center, Iran University of Medical SciencesIran; email: fam-biotec@iums.ac.ir
Uncontrolled Keywords: bone tissue engineering endothelial cells gelatin/bioactive glass in vivo nanocomposites Biocompatibility Bone Cell engineering Cytology Defects Glass Laminating Pore size Rats Tissue Calvarial defects Critical sized defects Glass nano-composites Histomorphometric analysis In-vivo Nanocomposite scaffolds Tissue-engineered bones Scaffolds (biology)
Subjects: WE Musculoskeletal system
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 11 May 2020 08:54
Last Modified: 11 May 2020 08:54
URI: http://eprints.mums.ac.ir/id/eprint/17358

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