The Effect of Allium cepa Extract on Lung Oxidant, Antioxidant, and Immunological Biomarkers in Ovalbumin-Sensitized Rats

Marefati, N. and Eftekhar, N. and Kaveh, M. and Boskabadi, J. and Beheshti, F. and Boskabady, M. H. (2018) The Effect of Allium cepa Extract on Lung Oxidant, Antioxidant, and Immunological Biomarkers in Ovalbumin-Sensitized Rats. Medical Principles and Practice, 27 (2). pp. 122-128.

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Abstract

Objectives: To evaluate the effects of Allium cepa (A. cepa) on levels of oxidants, antioxidants, and immunological markers in bronchoalveolar lavage fluids (BALF) of sensitized rats. Materials and Methods: Oxidant/antioxidant markers and cytokines in BALF of control rats treated with saline (group C), ovalbumin-sensitized rats (group S), rats treated with 1.25 μg/mL dexamethasone and 3 doses of A. cepa extract (35, 70, and 140 mg/kg body weight BW/day) (S + AC) were investigated. Comparison of the results between groups was performed using analysis of variance with the Tukey-Kramer post hoc test. Results: The oxidant markers nitrogen dioxide (NO2), nitrate (NO3 -), and malondialdehyde (MDA), and immunological markers interleukin (IL)-4 and immunoglobulin E (IgE) were significantly higher, but the antioxidant markers superoxide dismutase (SOD), catalase (CAT), thiol, and interferon (IFN)-γ, and the IFN-γ/IL-4 ratio were lower in sensitized rats compared to control rats (p < 0.001 to p < 0.01). Compared to group S, the levels of the following markers were significantly lower: NO2, NO3 -, and IgE in groups treated with the A. cepa extract, MDA and IL-4 levels in groups treated with 70 and 140 mg/kg BW/day of the A. cepa extract, and all these markers as well as IFN-γ in rats treated with dexamethasone (p < 0.001 to p < 0.05). However, there were significantly higher levels of SOD and CAT and an increased IFN-γ/IL-4 ratio (groups treated with 70 and 140 mg/kg BW/day of the A. cepa extract), and levels of thiol and IFN-γ (group treated with 140 mg/kg BW/day of the A. cepa extract) as well as SOD, CAT, and thiol (dexamethasone-treated group) versus group S (p < 0.00 to p < 0.05). Conclusion: A. cepa showed antioxidant and immunomodulatory properties in sensitized rats. © 2018 The Author(s) Published by S. Karger AG, Basel.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: MPPRE Correspondence Address: Boskabady, M.H.; Neurogenic Inflammation Research Center, Mashhad University of Medical SciencesIran; email: boskabadymh@mums.ac.ir
Uncontrolled Keywords: Allium cepa Antioxidants Asthma Immunomodulation catalase dexamethasone gamma interferon immunoglobulin E interleukin 4 malonaldehyde nitrate nitrogen dioxide onion extract ovalbumin superoxide dismutase thiol antioxidant biological marker interferon nitric acid derivative oxidizing agent oxidoreductase thiol derivative animal experiment animal model animal tissue antioxidant activity Article bronchoalveolar lavage fluid controlled study drug dose comparison male nonhuman onion oxidative stress rat sensitization analysis of variance animal comparative study immunology lung metabolism Wistar rat Animals Biomarkers Interferons Interleukin-4 Malondialdehyde Nitrates Onions Oxidants Oxidoreductases Rats Rats, Wistar Sulfhydryl Compounds
Subjects: WF Respiratory System
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 09 May 2020 05:54
Last Modified: 09 May 2020 05:54
URI: http://eprints.mums.ac.ir/id/eprint/17387

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