Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation

Mojtabanezhad Shariatpanahi, A. and Rokni, P. and Shahabi, E. and Varshoee Tabrizi, F. and Kerachian, M. A. (2018) Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation. BMC Research Notes, 11 (1).

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Abstract

Objective: In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR. Results: Using QIAamp DNA Blood Mini kit, nearly 27 (380 bp) to 35 (173 bp) cfDNA was recovered with a higher recovery of smaller size cfDNA (173 bp) in comparison to larger ones (380 bp). These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method. © 2018 The Author(s).

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Kerachian, M.A.; Cancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterIran; email: amin.kerachian@mail.mcgill.ca
Uncontrolled Keywords: Cell-free DNA Liquid biopsy Plasma cell free nucleic acid adult blood female human isolation and purification pregnancy procedures standards validation study Cell-Free Nucleic Acids Humans
Subjects: QY Clinical Pathology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 08 May 2020 15:26
Last Modified: 08 May 2020 15:26
URI: http://eprints.mums.ac.ir/id/eprint/17415

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